1902.] The Interrelationship of Variola and Vaccinia. 



127 



ment. They were fed and otherwise attended to by a man specially 

 detailed for the purpose. All instruments employed for the vaccina- 

 tion of monkeys, calves, or children were previously sterilised by 

 boiling or passing through the flame of a spirit lamp. Attention may 

 perhaps be called to the fact that the skin of the scrotum in the calf 

 affords a specially favourable site for inoculation experiments, especially 

 if, when the incisions are made, the skin is made tense by pressing down 

 the testicles. The first transference from the monkey to the calf does 

 not usually afford a perfect result. Indeed a second, third, or even 

 later passage from calf to calf was usually required before the most 

 typical vesiculation was obtained. 



In certain cases children were vaccinated with lymph obtained from 

 the experimental calves, and in all instances the resulting vaccination 

 ran a perfectly normal course. With lymph of similar origin I also 

 successfully vaccinated myself. But none of the strains of vaccine 

 lymph, derived originally from human small-pox in the manner 

 described, have been brought into general use. 



PROTOCOLS OF EXPERIMENTS. 

 Ftest Sebees. 



February 21, 1898. — Grlycerinated samples of small-pox lymph, received this day 

 from Medical Officer of Health of Middlesbrough. Patients living and aged 

 respectively 20, 27, and 34 years ; all had been vaccinated in infancy. 



April 1. — At Brown Institution, inoculated small rhoesus monkey with small- 

 pox emulsion of 21.ii.98 in five linear incisions on left arm, and in fourteen on 

 abdomen, after pi-evious shaving and cleansing of the skin, by means of soap and 

 water, followed by warm boric acid lotion. Monkey isolated in separate room 

 and attendant vaccinated as a precautionary measure. 



April 5. — All insertions on both arm and abdomen evidently " taking." 



April 8. — Distinct vesiculation at site of all incisions on arm and most of those 

 on abdomen. Monkey aetherised and substance of vesicles removed with sharp 

 spoon into small previously weighed and sterilised test-tube. Scrapings weighed 

 (0'6 gramme) and ground up with six times ttie weight of 50 per cent, watery 

 solution of glycerine. Resulting emulsion taken up into twelve capillary glass 

 tubes. 



April 13. — Monkey looks well. All incisions healed up. No sign of generalised 

 eruption. 



Calf Experiments. 



April 9. — At the Animal Yaccine Establishment, Mr. Stott inoculated Calf No. 1 

 (No. 4363) on two scarified patches, in twelve incisions on scrotum, and forty-four 

 in perineum and on abdomen, with contents of two capillary tubes of glycerinated 

 pulp prepared from vesicles of monkey. Incisions made with scalpel previously 

 sterilised. 



April 12 (72 hours). — Practically nothing to be seen. 



April 14 (120 hours). — All insertions on scrotum appear to have "taken," 

 and, in addition, four (not quite so well) on abdomen. Insertions on perineum 

 seem to have failed. Large bullous -looking vesicle on upper scarified patch. 



