128 



Dr. S. Monckton Copeman. 



[Nov. 13, 



From this and from the vesicular lines on scrotum and abdomen collected pulp by 

 scraping, after clamping with compression forceps. 



The same day (April 14), Calf No. 2 (No. 4369 in A.Y.E. records) was 

 inoculated on perineum, scrotum, and abdomen with material obtained from Calf 

 No. 1. 



April 19. — All inoculated lines, with exception of two on abdomen, '''taken" 

 well, eruption being markedly vesicular. Vesicles clamped and scraped ; pulp 

 being immediately employed for inoculation of Calf No. 3 (No. 4673 in A.V.E. 

 records) in a number of long incisions on the perineum, scrotum, and abdomen. 



April 24. — All lines of incision "taken" well j eruption perfectly typical of 

 vaccinia. 



From this calf, six children vaccinated at A.V.E. same day. 



May 1. — Children returned for inspection, in ordinary course. All vaccinations 

 completely successful. Photographed arms of two of these children, which pre- 

 sented most perfect eruption. 



About a month later I hunted up the parents of all six children, when I learnt 

 from the mothers' statements that in every case the vaccination had pursued a 

 perfectly normal course. 



Second Series. 



March 3, 1900. — At the West Ham Borough Hospital, Dagenham, I removed 

 small-pox vesicles from body of a man, eet. 56, who had died 24 hours previously 

 from semi-confluent form of disease. Material removed in test-tube and placed 

 in ice- chest. 



March 5. — After removal of some shreds of epithelium for histological pur- 

 poses, the remainder (0"25 gramme) ground up with twice its weight of 50 per 

 cent, solution of glycerine, and the greater portion stored in amber-coloured 

 capillary tubes. The test-tube was afterwards swabbed out, and cover-glass speci- 

 mens made for microscopical examination. 



With some of the glycerinated emulsion inoculated, at the Brown Institution, 

 rhtesus monkey (young female) on shaved area of back, about 3 inches by 2 inches, 

 previously well washed with warm boric acid solution. Incisions twelve in 

 number made " en 6chelon." 



March 8. — Inoculation has evidently " taken," as tips of each incision are 

 distinctly raised, and whole prospect is that of a typical calf vaccination of about 

 same age (72 hours). 



March 12. — Eruption beautifully perfect ; edges of vesicular portion a little 

 irregular, and centre of each line of incision occupied by commencing " crust." 

 No general eruption visible. Two photographs taken. 



Removed lower half of vesicular area with sharp spoon (upper portion left in 

 order to watch further development), and glycerinated resulting pulp. Material 

 used for inoculating Monkey No. 2 and also a calf (No. 606). 



March 12. — Monkey No. 2 inoculated in fourteen incisions with glycerinated 

 pulp from Monkey No. 1. Technique as before. 



March 19. — Eruption not so perfect as in Monkey No. 1. Vesicles not so 

 defined and plump. Monkey very wild, and has made sites of incisions bleed by 

 dashing from side to side of cage, which may be in some degree the cause. 

 Photograph taken. Vesicular pulp removed and ground up with four times its 

 weight of dilute glycerine. With some of this emulsion MonJcey No. 3 (young- 

 male rhcesus) inoculated same day in ten linear incisions on shaved area of 

 back. 



March 26. — All insertions "taken" well. Photographed. Material collected 

 and glycerinated, pulp being diluted about fifteen times, by mistake. Some used 



