1902.] The Inter-relationship of Variola and Vaccinia. 129 



for inoculation in twelve insertions of Monkey No. 4 (young male rhoesus) same 

 day. 



April 2.— All places have "taken" well, although material used for inoculation 

 had been so diluted. Photographed. Vesicles scraped and pulp glycerinated. 



Calf Experiments. 



March 12. — Inoculated Calf No. 1 in half dozen long linear incisions by method 

 usually employed in current vaccinations at Government Establishment, with 

 glycerinated pulp from Monkey No. 1. 



March 16. — Lines of incision slightly raised and red. 



March 17. — Dr. Fremlin found few small vesicles had developed. These he 

 clamped, inserting material obtained thereby on Calf No. 2. 



March 21. — There were evident signs of "taking" at all po nts of insertion, 

 the lines of incision being elevated and with a tendency to vesiculation. But 

 appearances not considered sufficiently typical to permit of material removed being 

 utilised for vaccination of children. 



At this point, owing to unforeseen circumstances, this particular series of 

 experiments was discontinued. 



Third Series. 



February 25, 1901. — At the Jenner Institute inoculated medium-sized macaque 

 monkey with small-pox emulsion, made by working up scrapings from P.M. cases 

 of the disease (received from Dr. Thomson, of the Belvedere Hospital, Glasgow), 

 in a small amount of pure glycerine. Technique as in previous experiments. 

 Incisions made on the monkey's hack with blunt scalpel, which had lost its temper 

 bj constant passing through the flame, so that all incisions did not apparently 

 penetrate to the true skin. 



March 4. — " Taken " well, though not throughout all insertions as in first 

 monkey of the last series, but as failure had only occurred where there was no 

 mark of incision, it was probably for reason mentioned above, as the eruption 

 -which had appeared was good. Not markedly vesicular ; lines of incision which 

 ■were covered with slight crust being surrounded by a pinkish papular eruption. 

 Photograph taken. 



Scraped with aid of compression forceps, and rubbed up material in small 

 amount of NaCl 0"7 per cent, solution. 



Monkey No. 2 inoculated this day, with emulsion of scrapings from Monkey 

 No. 1. Technique as before. 



March 16. — All insertions " taken " ; slightly more vesicular than in No. 1. 

 Photographed. Compression forceps applied and scrapings removed and rubbed 

 up in small mortar with NaCl solution. 



Monkey No. 3 inoculated immediately, on the back, with emulsion of material 

 obtained from No. 2. Animal very young ; hair downy and not easily shaved. 



March 18. — All insertions have " taken " and have wide whitish vesicular 

 margin, but appearance rather spoiled as hair on back has grown so rapidly. 

 Lymph oozed up when compression forceps applied. Photographed. Scrapings 

 rubbed up with NaCl solution, of which small quantity was used immediately for 

 inoculation of Monkey No. 4. Remainder glycerinated, tubed and stored in ice- 

 chest for future trial on calf. 



March 23. — Monkey No. 4, an old animal, had not apparently taken as well as 

 No. 3, so series was discontinued. 



April 20. — Monkey No. 3 vaccinated in six incisions on outside of thigh with 

 •current vaccine lymph of known potency. 



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