130 



Dr. S. Monckton Copeman. 



[Nov. 13, 



April 27. — No result, although monkeys not previously protected take vaccinia as 

 successfully as in the human subject.* 



Calf Experiments. 



March 22. — Calf No. 1 (1332), at the Government Animal Yaccine Establish- 

 ment, inoculated with small quantity of glycerinated emulsion of vesicular pulp 

 from Monkey No. 3. 



March 27. — Tiny papules and vesicles which had made 'their appearance along 

 lines of incisions removed with Yolkrnann's spoon and glycerinated. 



March 29. — Material collected on March 27 inserted into three long incisions on 

 Calf No. 2 (1342). 



April 3. — Fifth day. Lines of all three incisions occupied by good vesicles, 

 Photograph taken by Dr. Green. Vesicle pulp (0 37 gramme) removed and 

 glycerinated. 



April 24. — Calf No. 3 (1390), inoculated (with portion of material collected on 

 April 3) in thirty-six incisions on abdomen and scrotum. 



April 29. — All insertions had "taken" well, vesicles surrounded with slight 

 pink areola. Vesicles scraped and pulp glycerinated. Emulsion stored in ice- 

 chest. 



October 3. — Four c.c. of this emulsion used for vaccination of calf at the Jenuer 

 Institute, by numerous linear incisions extending nearly whole length of abdomen, 

 after manner usually employed at Government stations. 



October 8. — Appearance indistinguishable from normal vaccination. 63 grammes 

 of vesicle pulp collected and glycerinated. 



The glycerinated emulsion prepared from material removed from the calf on. 

 October 8 was subsequently employed for the vaccination of other calves, a strain 

 of lymph being thus obtained which continued to give excellent results both on 

 children and calves. But the strain was never brought into general use, and all 

 the glycerinated emulsion remaining was eventually destroyed. 



Fourth Seeies. 



April 29, 1901. — At the Jenner Institute young rhcesus monkey shaved on back, 

 as in previous experiments, and inoculated in a dozen linear incisions with glyceri- 

 nated emulsion of S.P. vesicle pulp received from Dr. Thomson, of the Belvedere 

 Hospital, Glasgow, on March 26, 1901. 



April 2 (120 hours). — Had " taken" so well that I decided to collect; lines of 

 incision distinctly vesicular. After taking photo, washed inoculated area, and 

 removed pulp with aid of clamp forceps. Thin lines of altered epithelium came 

 off as in a good calf vaccination. No " crusting." 



Monkey No. 2 inoculated same day with material obtained as above and subse- 

 quently triturated in small glass mortar with small quantity of equal parts of 

 glycerine and normal 1 saline solution. 



May 9(120 hours). — Every insertion "taken" successfully. More markedly 

 vesicular in places than Monkey No. 1. Photograph taken, followed by usual 

 process of collection and glyceri nation af vesicle pulp. 



Monkey No. 3 inoculated on back in eight diagonal incisions. Emulsion remain- 

 ing over taken up into capillary tubes, of which two given to Dr. Fremlin for trial 

 on calf at A.V.E. on May 15. 



May 13 (120 hours). — Eruption of perfectly vesicular character along course of 

 all incisions made, the centre in each instance being occupied by thin linear crust. 



* Copeman, 'Journal of Pathology and Bacteriology,' May, 1894. 



