1902.] The Inter-relationship of Variola and Vaccinia. 



131 



Drs. Blaxall and Frernlin, on seeing the animal, both described eruption as being 

 equal to that which in case of calf vaccination -vould be entered in official records 

 as v.g. (very good). Monkey photographed and vesicle pulp collected and glyceri- 

 nated. Portion of emulsion used same day for inoculation of Monkey No. 4. 

 Another portion handed over to Dr. Fremlin for trial on calf at A.Y.E. 



May 18. — Monkey No. 4 "taken "well. Yesicle pulp collected, emulsified, 

 tubed and stored in ice-chest. 



Series not continued beyond this stage, as laboratory man had failed in attempts 

 to obtain further supply of young rhcesus monkeys. 



Calf Experiments. 



May 8. — Emulsion of vesicle pulp from Monkey No. 1, used at A.Y.E. for 

 inoculation of Calf No. 1418. 



May 13. — Dr. Blaxall noted " No vesiculation, slight thickening in one line." 



May 15. — Material from Monkeys No. 2 and No. 3, inoculated on calves at 

 A.Y.E. by Dr. Fremlin. 



May 20. — Both calves had " taken " to a certain extent, the result being most 

 marked in calf inoculated from Monkey No. 3. Material collected, glycerinated, 

 and stored. Portion subsequently handed to Medical Director of Jenner Institute 

 for further trial. 



May 26. — Inoculated calf at A.Y.E. with emulsion of second removal fron 

 Monkey No. 3. 



May 31. — Perfect vesicular eruption along course of all incisions. General 

 effect indistinguishable from that obtained with the current vaccine lymph of the 

 Government Establishment. 



In view of successful results following on vaccination of children with former 

 lymph, stocks raised in similar fashion, it appeared unnecessary to employ this 

 particular lymph for infantile vaccinations. But on vaccinating my own arm with 

 it direct from the calf, I succeeded in raising by the eighth day a fairly typical 

 vesicle, an effect in excess of that obtained by me on my own person at previous 

 attempts at vaccination. 



No further transference of this lymph was attempted. 



My first series of experiments bad not long been concluded, when 

 I came across a reference to an account of similar work which had 

 been carried out by Dr. Eilerts de Haan. The reference occurred in 

 a paper by Dr. Bruno Galli-Valerio,* and on hunting up Dr. de Haan's 

 original paper, which is entitled, "Vaccine et Retrovaccine a Batavia," f 

 I found that it contained an account of a lengthy series of experi- 

 ments on the variolation of monkeys and on the transference of the 

 resulting affection to calves. 



Dr. de Haan's work proved of special interest to me for the reason 

 that, quite independently, we had been able to corroborate one 

 anothers' work, except as regards the transference of the strain of 

 variola vaccine to the human subject — a final test which Dr. Eilerts 

 de Haan did not, as he says, feel justified in attempting, in view of the 

 unfortunate experience of Chauveau, in connection with his abortive 



* ' Centralblatt fur Bakteriologie,' March 28. 189?, p. 380 et seq. 

 f < Annales de l'Institut Pasteur,' 1S96, p. 169. 



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