Table I.— Exp. 47. Seedlings of Hrumits secalinus, sown in coarse cleaned Sand in Beakers, to which doses of Nutritive Salts were added on July 7, 1902. On July 23, infected 1st and 2nd Leaves with Spores from lirumu.t sywirrvwi/s. 



Eip. No. 



Number of 



seedlings. 



Distilled trader. . . 



MinUS pllO-phoi'U! 



,, calcium .. 



,, magnesium 



„ nitrogen . . 



,, potassium 



Normal 



P-free + Fe . . . . 

 Ca-free + Fe 

 Mg-f reo + Fo . . . 

 N-frce + Fe . . . . 

 K-f ree + Fe . . . . 

 Normal + Fa . . . 

 Howe-dnne 



Number 

 inoculated. 



Condition of plants. 



Results 



on 

 July 31. 



Results on 

 August 1. 



Results on 

 August 3. 









Results on 



August 5. 





Stature. 



Robustness. 



Colour. 



Average 

 number 

 of leaves. 



Number of 

 seedlings 

 infected. 



Number 

 infected on 

 1st leaf. 



Number 

 infected on 

 2nd leaf. 



Number 



both 1st and 

 2nd leaf. 







I 



10—12 cm. 



Small 



Rather pale 



2 









Flecks ? 



One second leaf 







1 















1 













narrow 









shows pustules 











3 



12—16 „ 



Medium 



Slightly pale 



2$ 









































narrow 



















3 



20—22 „ 



Stout 



Crood green 



3 













One second leaf 



^ 



y 



1 















broad 









shows pustules 











3 



One = 9 em., 



Slight 



Fair, but tips 



2£ 













One first and two 



2 



1 



2 



1 





others 



of leaves 



medium 









second leaves show 













= 16—18 cm. 





dying off. 











pustules 











3 



Two =5—6 cm. 



Slight 



Pale 



2—3 

















1 



1 













Two = 14—15 „ 





narrow 





















One = 1 cm. 

























4 



16—18 em. 



Stout 



Good green 



3 



Flecks ? 



One first leaf and 



Two first and three 



4 



4 



4 



4 









medium 







three second leaves 



second leaves pus- 

























have pustules 



tuled 











3 



19—21 cm. 



Stout 



Bright green 



3£ 



Flecks ? 



One first and one 



Three first and two 



3 



3 



3 



3 









broad 







second leaf have 



second leaves show 

























pustules 



pustules 











5 



12—16 „ 



Medium 



Slightly pale 



21 

















4 



3 



3 



2 





























g 



20—22 „ 



Stout 



Vivid green 



3 



Fl 



scks? 



Two first leaves and 



Three first and five 



5 



4t 



5 



4 









broad 







four second leaves 



second leaves show 

























pustuled 



pustules 











4 



16-18 „ 



Slight 



Good green 



2i 









Flecks ? 



Two second leaves 



3 



2 



3 



2 









but tips 



rather 









pustuled 

















dying off 



narrow 



















4 



10—12 „ 



Small 



Good green 











Flecks ? 



Three first and three 



3 



3 



3 



3 









narrow 









second leaves pus- 



























tuled 











4 



16—18 „ 



Stout 



Good green 





Flecks ? 



Flecks ? 



One first and three 



4 





3 



1 











medium 









second leaves pus- 



























tuled 











3 



19—21 „ 



Large 



Deep green 





Flecks ? 



Two first leaves pus- 



Three first leaves 



3 



2 





2 







broad 







tuled 



show pustules 











6 



16-17 „ 



Stout 



Good green 





Flecks ? 



Three first leaves and 



Five first and three 



6 





6 









and 



broad 







one second leaf 



second leaves have 















coarse. 











pustuled 



pustules 











Remarks as to condition at end of 

 experiment.t 



Two or three small patches of small pustules, 

 yiolding fair spores. Leaf pale in infected 

 area. 



No trace anywhere of pustules. 



Only ono small group of small pustules, 

 rathor better than in a. Leaves green. 



Small but fairly well-developed pustules of 

 spores on one plant; only one pustleonthe 

 other. Leaves very pale and tips dead. 



Ono very minute pustule on pale first leaf. 



Extensive infection, und several patches 

 show strong pustules. Leaves rich green. 



Excellent crop of spores on lnrge pustules, 

 and leaves rich green. 



Minute pustules, difficult to detect and 

 bearing but few spores. Colour fuir. 



Very good pustules with abundant spores, 

 but few and small areas or patches of 

 infection. Leaves good green. 



Medium-sized patches of small pustules with 

 not many spores. Pale, and tips dead. 



Attacked area pale. Pustules small and few, 

 and yield few spores. 



Few but medium-sized pustules. One leaf 

 has a large patch. Green. 



Very large areas of woll-developed pustules, 

 rich in spores. Leaves deep green. 



All pustules largo and rich in spores, but 

 areas of leaf uround pale ; otherwise deep 

 green. 



On July 18 a dose of the K-free solution was given to this by mistake, consequently it received all salts, but i 



different proportion 

 X First 



from the normal. t On August 23 spores were taken from 

 leaf dead in one plant also. 



those plants and infections made on test-tube cultures of Bromus mmKmm (sec Table II). 



