1003.] Trypanosoma present in Cases of Sleeping Sickness. 501 



On the Discovery of a Species of Trypanosoma in the Cerebro- 

 spinal Fluid of Cases of Sleeping Sickness." By Aldo 

 Castella.ni, M.D. Communicated by the Malaria Committee 

 of the Eoyal Society. Dated " Entebbe, Uganda, 5th April, 

 1903." Eeceived May 8— Eead May 14, 1903. 



On the 12th November, 1902, when examining a specimen of 

 "Cerebrospinal fluid taken by lumbar puncture during life from a well- 

 marked case of sleeping sickness, I was surprised to observe a living 

 trypanosoma. Since that date I have made as many observations in 

 this direction as possible, and the results are to my mind sufficiently 

 surprising to excuse me for presenting this preliminary note. 



These trypanosomes are not in large numbers, so that to find them 

 it is necessary to draw off at least 15 c.cs. of the cerebro-spinal fluid. 

 It is better to reject the first few c.cs. as they are apt to contain blood. 

 When the fluid comes away clear, 10 c.cs. are collected and centrifuged 

 for 15 minutes. At the end of this time there is found at the bottom 

 of the tube a slight deposit of whitish sediment, and in some cases also 

 a minute trace of blood. 



The liquid above the sediment is poured off and the sediment 

 examined under a moderately low power of the microscope. As the 

 .trypanosomes are at first fairly active they are easily detected. 



The following tables represent the results of this investigation : — 



