170 Mr. E. H. Acton. The Assimilation of Carbon 



B. Solution supplied to the Roots. 



Solution used. 



Plants. 



Results. 



The culture solution 

 + 1 per cent, inu- 

 lin 



Acer pseudoplatanus 

 Cheiranthus Cheiri 



Starch was formed in the 

 leaves at the end of 5 

 days (not tested earlier) . 



No. 10. Experiments with " Soluble Starch" 



The starch solution was prepared by pouring starch (wheat- starch) 

 rubbed into a thin paste with water (cold) into an excess of boiling 

 water and boiled for five minntes ; on cooling the solution was filtered 

 through paper and diluted till the strength was about 1 per cent.* 



A. With Cut Branches. 



Solution used. 



Plants. 



Eesults. 



The culture solution 

 + about 1 per cent, 

 starch (soluble) 



Acer pseudoplatanus 

 Tilia Europcea 



Starch grains formed in 

 the leaves after 24 hours, 

 abundant after 48 hours. 



Ditto after 48 hours ; not 

 so abundant as above ; 

 increased after ti 

 days. 



B. Supplied to Roots. 



Solution used. 



Plants. 



Eesults. J r 



(a.) 



Same solution as in 

 10 A 



Acer pseudoplatanus 

 Epilobium hirsutumf 

 Phaseolus rnultiflorus 

 Tilia Europcea 



$ 



No starch formed v.- 



days) . 

 Ditto. 

 Ditto. 



* Sachs' method being obviously inapplicable in this case, micro-chemical obser- 

 vations on sections were used to detect the starch grains in the leaves. 



f The two seedlings of E. hirsutum used were apparently injured in a\ ^ing the 

 additional soluble starch, as they withered during the second six days,* ' As the 

 results were negative in other cases, this experiment was not repeated. 



Except in case of E. hirsutum (see note above), the plants all resumes, wth 

 and formed starch afte*- several days when planted out. 



