1904.] Conjugation of Besting Nuclei in Epithelioma of Mouse. 77 



" Conjugation of Besting Nuclei in an Epithelioma of the Mouse." 

 By E. F. Bashford, M.D., and J. A. Murray, M.B., B.Sc. 

 Communicated by Professor J. Kose Bradford, E.E.S. 

 Keceived January 28, — Eead February 4, 1904. 



In a previous communication we have drawn attention to the fact 

 that the power of cell proliferation, which has been proved to occur in 

 an epithelioma of the mouse (Jensen), is a phenomenon unparalleled 

 in the mammalia. A mass of tumour, 16 lbs. in weight, has been 

 produced by artificially transplanting portions of the original growth 

 and its descendants. In seeking to throw light on this fact, we have 

 studied carefully the phenomena which follow the transplantations of 

 portions of the tissue to new sites, and have found that the tumours 

 which arise are the genealogical descendants of the cells introduced. 

 We have studied the growth of the tumours which arise at successive 

 stages of 24 hours. In a tumour removed on the eighth day, and 

 less than half a split pea in size, conjugation of resting nuclei has been 

 observed. To take a specific case, the nuclei of two adjacent cells are 

 continuous through the cell wall by a tube-like bridge, in the middle 

 of which a strand of nucleolar substance with fusiform swellings in 

 either cell is visible. The cells of this particular case are adjacent to 

 the stroma, and close to the outer surface of the young tumour. 



