130 



Dr. A. E. Wright and Capt. S. E. Douglas. [Jan. 11, 



B. 



Washed corpuscles from normal man 3 vols. 



Serum from normal man 3 



Suspension of staphylococcus culture 1 vol. 



Phagocytic index (15 P.W.B.C.), 13. 



C. 



Immunised patient's washed corpuscles 3 vols. 



Serum from normal man 3 „ 



Suspension of staphylococcus culture 1 vol. 



Phagocytic index (15 P.W.B.C.), 13. 

 D_ 



Washed corpuscles from normal man 3 vols. 



Serum from immunised patient 3 „ 



Suspension of staphylococcus culture 1 vol. 



Phagocytic index (15 P.W.B.C.), 28-2. 



Experiments on the Opsonic Power of Human Blood in its 

 Eelation to the Bacillus of Plague. 



In these and all subsequent experiments, unless where otherwise 

 specified, the technique employed was exactly the same as that 

 employed in the experiments set forth above. It may further be 

 premised that the bacterial suspensions employed were in each case 

 suspensions of very young agar cultures — in most cases 24-hour 

 cultures — in physiological salt solution. By the term " heated serum " 

 is in each case to be understood serum which has been subjected to a 

 temperature of 60° C. for 10 minutes or more. 



Experiment 1. 

 A. 



S. B. D.'s unheated serum 3 vols. 



S. B. D.'s washed corpuscles 3 „ 



Suspension of plague bacillus , 1 vol. 



Phagocytic index (20 P.W.B.C.), 3'0. 

 B. 



S. B. D.'s heated serum 3 vols. 



S. B. D.'s washed corpuscles 3 



Suspension of plague bacillus 1 vol. 



Phagocytic index (25 P.W.B.C.), 0-7. 



