1904.] Role of Blood Fluids in connection with Phagocytosis. 137 



All these points must be taken into consideration in connection with 

 the subjoined experiments : — 



Experiment 1. 

 A. 



S. E. D.'s unhealed serum 3 vols. 



S. E. D.'s washed corpuscles 3 



Suspension of the cholera vibrio 2 



Everywhere considerable phagocytosis. Complete spherulation of 

 almost all the micro-organisms within and all the micro-organisms 

 outside the cells. No indication of vacuolation round the ingested 

 bacteria (fig. 3). 



Phagocytic index (14 P.W.B.C.), 24 (circ). 



B. 



S. R. D.'s heated serum 3 vols. 



S. E. D.'s washed corpuscles 3 „ 



Suspension of the cholera vibrio 2 „ 



Everywhere considerable phagocytosis. No spherulation of the 

 micro-organisms either within or without the leucocytes. Very marked 

 vacuolation of the leucocytes round the ingested bacteria (fig. 4). 



Phagocytic index (11 P.W.B.C.), 26'2 (arc). 



Experiment 2. 

 A. 



A. E. W.'s unheated serum 3 vols. 



S. E. D.'s washed corpuscles 3 „ 



Suspension of the cholera vibrio 1 vol. 



Complete spherulation of all the bacteria, whether within or without 

 the cells. 



Phagocytic index (21 P.W.B.C.), 8-1 (circ), 

 B. 



A. E. W.'s heated serum 3 vols. 



S. E. D.'s washed corpuscles 3 „ 



Suspension of the cholera vibrio 1 vol. 



No spherulation of the micro-organisms, either within or without the 

 leucocytes. 



Phagocytic index (13 P.W.B.C.), 0-8. 



L 2 



