138 



Dr. A. E. Wright and Capt. S. K. Douglas. [Jan. 11, 



Experiment 3. 



A. 



S. E. D.'s unheated serum 2 vols. 



S. R. D.'s washed corpuscles 2 ., 



Broth culture of the typhoid bacillus 2 „ 



Much phagocytosis. Complete spherulation of all the extracellular 

 micro-organisms. Many of the bacilli in the interior of the leucocytes 

 have completely preserved their original contours, others — probably 

 the later ingested ones — are spherulated (fig. 1). 



B. 



S. B. D.'s heated serum 2 vols. 



S. R. D.'s washed corpuscles 2 „ 



Broth culture of the typhoid bacillus 2 



Much phagocytosis. All the micro-organisms, whether within or 

 without the leucocytes, are morphologically unaltered and have pre- 

 served their staining properties unimpaired (fig. 2.). 



Experiment 4. 

 A. 



A. E. "W.'s unheated serum 3 vols. 



S, R. D.'s washed corpuscles 3 



Broth cultivation of the typhoid bacilhis 2 



Complete spherulation of all the extracellular bacteria which have 

 escaped solution. In interior of leucocytes most of the bacteria have 

 undergone spherulation, but in the centre of the corpuscles some — 

 probably those which were soonest ingested — are morphologically 

 unaltered and preserve their staining properties unaltered. 



Phagocytic index, 100 (estimated). 



B. 



A. E. "Vy.'s heated serum 3 vols. 



S. R. D.'s washed corpuscles 3 



Broth cultivation of the typhoid bacillus 1 vol. 



No spherulation, either within or without the cells. 



Phagocytic index (20 P.W.B.C.), 31-8 (circ). 



Experiment 5. 



A. 



S. R. D.'s unheated serum. 3 vols. 



S. R. D.'s washed corpuscles 3 



Suspension of the typhoid bacillus 1 vol. 



All the bacilli both within and without the cells have undergone 

 spherulation. 



Phagocytic index (11 P.W.B.C.), 13-6. 



