342 Dr. A. B. Green. On some Additional Points in [Apr. 19, 



be so much shattered and can be used two or three times before 

 repolishing. 



I made special tests with a copper bead and copper plate, but there 

 was no change in results. Also using a copper point and copper 

 plane the gradient came out about half as much as indicated in the 

 table above. Again, when using a bead and disc, it is found to be 

 immaterial which is -positive and which negative, so that for small 

 distances there does not appear to be a different positive and negative 

 tension as was found by Faraday for large distances. 



I hope to continue the research, varying the conditions. My best 

 thanks are due to the Eoyal Society for grants in aid of research 

 extending over the last three years. 



I wish also to express my great obligation to Professor W. H. Heaton 

 for granting me every facility in my investigation. 



" Further Note on some Additional Points in Connection with 

 Chloroformed Calf Vaccine." By Alan B. Green, M.A., 

 M.D. (Cantab.). Communicated by Dr. W. H. Power, C.B., 

 F.E.S. Beceived April 19 —Bead May 5, 1904. 



(From the Grovernment Lymph. Laboratories.) 



Since April, 1903, the date of my preliminary note* on this subject, 

 the preparation of calf vaccine by the chloroform process has been 

 carried on in at these laboratories and a large number of vaccines have 

 now been treated by this method. These lymphs have been freed from 

 their non-sporebearing extraneous bacteria within a period ranging 

 between 1 and 8 hours after their collection from the calf, and 

 have, subject to the usual tests, been issued for general vaccination 

 purposes about 2 weeks after collection. Their use has resulted in 

 high " case " and " insertion " success. 



The following points in connection with these vaccines have been 

 investigated. 



The Effect of Temperature in the Elimination of Extraneous Micro-organisms 

 from Crude Calf Vaccine by the Chloroform Process. 



It has been ascertained that the temperature at which vaccine 

 emulsion is subjected to the chloroform process determines largely the 

 rate at which the extraneous bacteria of that emulsion are eliminated. 

 This has been shown in two ways. 



* ' Koy. Soc. Proc.,' 1903. 



