410 



Prof. B. Moore and Mr. H. E. Eoaf. [Apr. 12, 



Experiment 7. — Solution of haemoglobin, of equal strength in 

 haemoglobin to blood from which obtained, and containing the 

 amounts of chloroform shown in the table. The results are shown 

 graphically by Curve 3 of fig. 6. 



Experiment 7. — Haemoglobin Solution. 



Temperature 40° C. 



by wciglit of 

 chloroform 

 originally 

 introduced. 



Irressure ot 

 chloroform in 

 vapour space 



in mm. of 

 mercury. 



Percentage 

 by weight of 



chloroform 

 pumped off 

 into rapour 

 space. 



Percentage 

 by weight of 



chloroform 

 remaining in 

 solution. 



Coefficient of 

 distribution 

 between 



vapour space 

 and solvent. 



0-02 



2 -85 



-0018 



-0182 



1 



10 -l 



0-04 



7-31 



-0045 



-0355 



1 



7-9 



-06 



14-52 



-0089 



-0511 



1 



6-6 



0-08 



17 '79 



-0110 



-0690 



1 



6-3 



o-i 



22-34 



-0138 



-0862 



1 



6-3 



0-2 



43-85 



-0270 



-1730 



1 



6*4 



0-3 



62-01 



-0382 



-2618 



1 



6-8 



0-4 



93 -15 



-0573 



-3427 



1 



5-9 



0-5 



114 -91 



'0707 



-4393 



1 



6-2 



0-6 



136 -47 



-0840 



-5160 



1 



6'2 



0-8 



197 -10 



-1213 



-6787 



1 



5 -6 



1 -o 



222 -64 



0-1371 



-8629 



1 



6-3 



1-5 



262 -26 



-1614 



1 -3386 



1 



8-3 



2-0 



268 -00 



-1650 



1 -8350 



1 



11 -1 



IV, — Solubilities of Gases in Serum and Haemoglobin in Presence of 

 Chloroform. 



It was thought that such compounds as are shown by the above 

 experiments to be formed between chloroform and serum or 

 haemoglobin solution, might interfere with the carriage of oxygen and 

 carbon-dioxide by the blood. Accordingly, experiments were carried 

 out upon serum and haemoglobin solutions to test this point. 



A volume of about 500 c.c. of serum or of haemoglobin solution obtained 

 as for the experiments in Section 3, was completely deprived of gases by 

 exhaustion with a Topler pump at 40° C, afterwards saturation with 

 air or air and carbon-dioxide, was carried out upon two equal volumes 

 contained in similar bottles to one of which a sufficient quantity of 

 chloroform was added to make a ]-per-cent. solution, while the other 

 served as a control. The gases dissolved in each case were then 

 collected by means of the Topler pump as before, and analysed. 



The results throughout were negative, and thus proved a fortiori 

 that at the anaesthetising values chloroform does not depress the 

 solubility of the respiratory gases in the blood. 



