1904.] 



Studies on Enzyme Action. 



509 



it was found that an approximately constant weight of sugar was 

 hydrolysed whatever the concentration of the sugar. 



Table XIV. — Amount of Sugar Hydrolysed. 



Solution 

 containing — 



22 hours. 



46 hours. 



70 hours. 



Propor- 

 tion. 



Weight. 



Propor- 

 tion. 



"Weight. 



Propor- 

 tion. 



Weight. 





22 



2-2 



29 



2-9 



39 -6 



3-96 



20 „ 



13 



2*6 



20 



4-0 



25 -1 



5-0 



30 „ 



10 



3-G 



15 



4-5 



19 -0 



4-7 



Table XY. — Amount of Sugar Hydrolysed. 



Solution 

 containing — 



23 hours. 



48 hours. 



92 hours. 



Propor- 

 tion. 



Weight. 



Propor- 

 tion. 



Weight. 



Propor- 

 tion. 



Weight. 



20 „ 



30 „ 



19 "7 

 10 -6 



7 -0 



1-97 

 2 -12 

 2 -10 



29 -8 

 15 -3 

 10-2 



2- 98 



3- 06 

 3 -06 



35 7 

 23 -3 

 16 -3 



3- 57 



4- 66 

 4-89 



The conclusions drawn from the experiments recorded in the three 

 preceding tables apply only to the concentrated solutions in which 

 the proportion of enzyme present was small ; in very dilute solutions, 

 on the other hand, quite another effect is produced by changing the 

 concentration. Table XVI relates to experiments in which the pro- 

 portion of enzyme present was large relatively to that of sugar. It 

 will be seen that on increasing the amount of sugar present there was 

 nearly a proportionate increase in the amount hydrolysed, though 

 the proportion hydrolysed, as well as the value of K, remained 

 constant, a result in agreement with the law of mass action. It should 

 be mentioned, however, that the error affecting titration in these 

 experiments is somewhat large, owing to the influence of the proteids 

 on the determination of the end point. 



Table XVI. 



Milk sugar per 

 100 c.c. 



Amount changed 

 in 3 hours. 



K. 



1 *0 gnis. 



0-1S5 



-0296 



0-5 „ 



0-098 



-0298 



0-2 „ 



-0416 



-0337 



