374 



Dr. C. A. MacMunn. 



1st Band \661— 646 



2nd „ A608— 589 



3rd „ X576— 561 or 558 



4th „ X539— 521 



5th „ \505— 484 



The colouring-matters were seen by the microscope to be deposited 

 in brown grannies, and no parasitic algas could be detected. 



In the yellow ether extract of the liver no band in red could be 

 detected, but two feeble bands, one at F, the other between F and G, 

 were visible, probably due to the presence of a lutein pigment. 

 Chloroform extracted no colouring-matter. 



Liver of Cardium edule* — The yellow alcoholic filtrate of the liver 

 gave a band in red, and a band between green and blue at F. That 

 in red extended from \678 — 656, but was difficult to read. With 

 nitric acid the liquid became greenish and gave the same series of 

 bands as before. 



Liver of Anoilontcb ajgnea. — When extracted with rectified spirit 

 and filtered a deep yellow solution formed, which showed three bands 

 as follows : — 



1st Band . . X684— 663 "5 1 



2nd „ X620— 600 f Sp. 4. 



3rd „ X555— 539 J 



Treated with nitric acid this solution became greenish and five 

 bands now appeared, which were difficult to read, but the first 

 extended from X678 — 656, and the fifth probably from A505 to 



484 (?) 



In the microspectroscope the bands appeared as shown in sp. 5. 

 In another specimen of Anodonta an orange-coloured mass was 

 found in the intestine, which gave two bands like those of reduced 

 heematin, and a third close before F, the latter extending from A-505 — 

 486. 



Liver of TJnio. — The appearances coincided with those just given 

 for Anodonta. 



All the extracts of the livers of the above Lamellibranchiates were 

 treated with ammonium sulphide with a negative result, and therefore 

 did not contain hasmochromogen. 



The colouring-matter of the liver of Anodonta was partially 

 soluble in ether with a yellow colour, and this solution showed 

 practically the same spectrum as the alcohol extract. 



Bile and Liver of Gephalopod Mollusca. Octopus. — The only cepha- 

 lopod which I had an opportunity of examining was Octopus vulgaris. 

 The yellow-brown liver became darker on exposure to air, due to the 



# Fasting for three days. 



