Colouring -matters of the so-called Bile of Invertebrates, fyc. 401 



a purple solution which, gave two bands : 1st, from A-630 — 589 ; 

 2nd, about \573 — 555. Hence this urine did contain indican. 



Although the spectroscopic characters of the second specimen did 

 somewhat resemble those of urrhodin, yet those of the first were quite 

 different, and hence T have concluded that the colouring-matter was 

 not urrhodin, It also differs from the pigment described by Plosz,* 

 and seems from the description of (l Urorosein " of Nencki and Sieberf 

 to resemble that pigment, which showed in amylic alcohol a band 

 between D and E with its maximum of shading at X557. It is at all 

 events closely related to urrhodin. Unfortunately I had not material 

 enough for further study. 



In the urine of progressive pernicious anaemia a peculiar colouring- 

 matter was noticed, which after boiling the urine with hydrochloric 

 acid and shaking with chloroform, imparted to the latter extract a 

 fine red colour, giving a band between D and E, but I hope to study 

 the pigments of the urine of that disease more fully at some future 

 time. 



EXPLANATION OF SPECTRUM CHART. 



Sp. 1. Alcohol extract of chlorophyll of Primula (obtained as described in the 

 paper) treated with nitric acid. 

 „ 2. Alcohol extract of liver of Ostrcea edulis, showing band of enterochlorophyll 

 in red at B. 



„ 3. The same solution with nitric acid, winch developes exactly the same spectrum 

 as 1. 



„ 4, Alcohol extract of liver of Anodonta cygnea. The greater breadth of band 



at B is due to fact that more chlorophyll is present than in the liver extract 



of which 2 is the spectrum. 

 „ 5. The same solution with nitric acid. The slight difference of position of 



the bands in this and spectra 1 and 3 is due probably to a little more, or 



a little less, acid having been added. 

 6. Alcohol extract of liver of Octopus vulgaris. This appears to give without 



any treatment a spectrum closely resembling 1, 3, and 5. 

 „ 7. Ether extract of the same liver, showing double band in red. In some cases 



this double band, or another like it, is got by treating enterochlorophyll 



with nitric acid. 



,, 8. Alcohol extract of liver of Buccinwn undatum. This, like the extract of liver 

 of Octopus, appears to contain acid enterochlorophyll ; ef.it with 6, 5, 3, 1. 



„ 9. Alcohol, extract of liver of Helix pomatia, showing band of enterochlorophyll 

 and those of ha3mochromogen (reduced hsematin) . 



„ 10. Bile of Limax jlavus, treated with a little ammonium sulphide, showing the 

 bands of reduced hsernatin. The slight difference in the position of the 

 latter and those of 9 is due to the solvent, alcohol in case of 9 and " bile " 

 in case of 10. 



„ 11. Bile of .Homarus vulgaris, showing presence of a pigment identical with one 



* " Zeitschrift fur Physiol. Chemie," Band VI, Heft 6, 1882. 

 f " Journ. Chemie," xxvi, 333 -336. 

 VOL. XXXV. 2 P 



