206 



form made its appearance in the cultures. Cultures derived from the 

 conidia of the Spicaria-ioxm reproduced the same form in four or five 

 days, and after some weeks the Fusarium-iorm appeared in small 

 quantity. Cultures derived from the Fusarium-iorm produced both 

 the Spicaria-iorm and the Fusarium-iorm simultaneously. Pure 

 cultures were obtained and used for inoculation. The results which 

 attended the progress of the growth in the cultures showed that 

 Nectria diversispora was possessed of two conidial forms and the 

 Nectria. 



The conidia of the Fusarium-iorm and the Spicaria-iorm were 

 obtained for inoculation from the pure cultures of which eighteen had 

 tetn prepared and studied. Three modes of inoculation were 

 employed ; one consisted in applying conidia and mycelium from a 

 culture on to the freshly tapped surfaces of healthy four-year-old 

 trees and keeping the surfaces moist for four days ; the second con- 

 sisted in removing small squares of the bark to the depth of the 

 latex layer, allowing the exposed tissues to remain for a few hours, 

 when any latex which had exuded from the wound was removed, 

 transferring conidia and mycelium to the exposed tissues and repla- 

 cing the square Of outer bark in position. The third consisted in 

 cutting by means of an oblique cut down to the wood on the one- 

 month-old and two-months-old tapping surface, and transferring 

 conidia and mycelium to the cut surfaces of the wood. In the two 

 last named methods the trees were healthy and were ten years of age; 

 control cuts were made on each tree and all of the cuts were kept 

 moist for six days. The ascospores were obtained by crushing ripe 

 perithecia in a small quantity of recently boiled distilled water and 

 transferring them to the wounds; they were employed for inoculation 

 in the second and third methods. Altogether eighteen trees were 

 employed, six being used for each of the methods described above ; 

 and in the second and third series each tree was inoculated at three 

 wounds with conidia of the Spicaria-iorm, with conidia of the 

 Fusarium-iorm and with ascospores of the Nectria, making nine 

 inoculations on each tree At the end of eight weeks the inoculations 

 were examined. In those of the first series no effect could be observed 

 on the tapping surface ; in those of the second series the wounds were 

 healing, and the squares of bark which had been replaced in the 

 cuts were being forced out by the healing of the wounds ; in those of 

 the third series the wounds were healing well. A further exami- 

 nation at the end of ten weeks failed to show any symptoms of 

 disease. It was concluded that the fungus was incapable of inocu- 

 lating either at the newly tapped surface or at a wound on the trunk. 

 These results serve to corroborate Fetch's conclusions as to the 

 harmless nature of the fungus. 



Stilhella Heveae"^ was described by Zimmermann from Java in 

 1902 on branches of Hevea. This fungus, or a species which in its 

 characters and measurements is indistinguishable from it, occurs 



* Renamed Stilbum Heveae Sacc. and D. Sacc. 



