32 



For the isolation of species, I have used Esmareh tilings or roll cultures 

 and the materials uuv taken from »o«»ns in different stages of the affection. 



Anion- the bacteria and fungi which have heen Isolated in this way, two 

 species of Aspergillus, A. glaucus and A. flavus, have lieen found invaria- 

 bly developed in the culture. 



On inoculating a number of unaffected cocoons with spores of these two 

 fungi taken l'r<>m toy pure cultures, 1 have found, after two weeks, tin • surface 

 of the cocoons presenting the characteristic yellowish hrown hue, owing to the 



We may, therefore, Uk up»n the cocoon fungus as formed chiefly of 



inner surface of the cocoou and esp<dally the ImK of the pupa within are 

 in most wises, affivted earlier than the outer surface of the cocoon, and also to 



Thus, the part first affected seems to be the body of the pupa rather 



open and the pupa examined. 



In this way, I found that the pupre to whose spiracles while in their 



