60 



REPORT OF THE COMMISSIONER OF AGRICULTURE. 



MANIPULATION. 



The butter fat is run out of a 5 c. c. pipette, as in tlie process for sat- 

 uration equivalent. Of the alkali solution 50 c. c. are used. Blanks 

 are made as before, except that the alcohol is evaporated off before the 

 titration is made. 



When the fat is saponified the contents of the bottles are washed into 

 large assay flasks. These are placed over the steam-bath and allowed 

 to remain until the alcohol has all been driven off. 



The amount of semi-normal acid necessary to saturate the alkali in 

 the blank tests is now accurately determined, and this amount is then 

 run into the flasks containing the soaps. Generally about 1 c. c. inoro 

 acid is added than is necessary to set free the fats j but this is not neces- 

 sary. 



Hot water is then added, 100-200 c. c, the flask stoppered (with a 

 long glass tube 1 m. in the stopper) and heated to boiling over the 

 steam bath with repeated shaking. The flask is then cooled (in ice- 

 water to hurry the analysis) and the water, as soon as the insoluble 

 acids have solidified, poured through a filter into a graduated flask 

 (500-1,000 c. c). This process is repeated three or four times and the 

 filtrate made up to 500-1,000 c. c., and 100 c. c. taken for titration with 

 deci normal alkali solution. If any excess of acid has been added it is 

 deducted from the total and the remainder is the soluble acid. It is 

 calculated as butyric acid by multiplying number c. c. alkali by .0088. 



The insoluble acids are brought into a tared dish, any in the filter oi 

 flask being dissolved in ether, dried at 100° with stirring with absolute 

 alcohol to remove water, and weighed. 



In the table will be found the results of the analyses. 



Triplicate and duplicate analyses of fatty acids in outters and substitutes. 



Soluble acid. 



Insoluble acid. 



No. 



1. 



2. 



3. 



Mean. 



No. 



L 



2. 



3. 



Mean. 



2 



5.14 



5. 14 



5. 10 



5.12 



1 



87. 43 



8R 09 



87. 91 



87. 81 



4 



5. 20 



5.12 



5. 12 



5. 14 





87. 88 



87. 73 



87.91 



87. 84 



6 



4.48 



4. 46 



4.63 



4. 52 



6 



88. 25 



87. 97 



87 S2 



88. 02 



1 



5. 57 



5.51 





5. 54 



20 



94. 81 



95. 13 



94. 46 



94. SO 



3 



5. 50 



5. 60 





5. 55 



24 



95. 33 



95.38 



95. 51 



95. 40 





4. 78 



4. 60 





4. 69 



1 



87. 70 



88. 02 





87. S6 



8 



5. 14 



4. 90 





5. 02 



3 



86.31 



86. 55 





86. 43 



9 



5. 07 



5. 14 





5.10 



8 



88.25 



87. 92 





S8. 08 



10 



4. 81 



4.76 





4. 78 





87. 53 



87. 54 





87. 54 



11 



5.10 



4. 91 





5. 00 



11 



88. 67 



88. 63 





88. 65 



12 



4. 90 



5.04 





4. 97 



12 



88. 59 



88. 34 





88. 46 



14 



4.49 



4.49 





4.49 



14 



88.89 



88. 82 





88. 85 



15 



4. 62 



4. 60 





4. 61 



15 



87. 98 



87. 04 





87. 51 



10 



5. 70 



5. 63 





5. 66 



16 



87. SO 



87. 52 





87. 70 



17 



4. 56 



4. 25 





4. 40 



17 



87.97 



87. 61 





87. 79 



18 



4. 39 



4. 91 





4. 60 



21 



93. 22 



93. 63 





93. 42 



19 



4. 38 



4. 82 





4.60 



22 



93. 70 



93. 60 





93. 65 



21 



.20 



.20 





.20 



23 



i 



94. 52 



95. 09 





94. 80 



22 



.56 



.56 





.56 





