264 report op the commissioner of agriculture. 



This was also suspended in salt solution and injected hypodermically in 

 the dose 2°*- to 3 CC - at the inner side of the thigh. 



To and including July 17, or for the first ten days, there were small, 

 hard swellings at|the point of inoculation, but no positive signs of disease, 

 and the appetite remained good. There were considerable variations 

 in the temperatures, but it is doubtful if this had any pathological sig- 

 nificance. Julv 18, three were evidently sick, with temperatures of 

 102p, 1051, andl06f° F. 



The one most severely affected was killed July 21, at which time the 

 temperature was 104J° F., and there was complete loss of appetite. 

 The point of inoculation was much swollen, the enlargement extending 

 forward under the abdomen, and was about 6 inches in length by 2 

 in breadth. When cujfc across it was found to be dense and fibrous and 

 creaked under the knife. A clear lymph flowed from the cut surface. 

 In the center of the swelling was an irregular cavity, 1 to 2 inches 

 across and partly filled with dry caseous material, reminding one of 

 the sequestrum formed in fowl cholera when an inactive virus is injected 

 into the muscles in large quantities. The right lung was nearly all of 

 a deep-red color with extensive areas of infarction. There was a small 

 quantity of effusion in the cavity of the thorax. The intestinal tract 

 was congested but there was no peritoneal effusion. 



The pleural effusion was collected in vacuum tubes with all known 

 precautions to prevent access of atmospheric germs, and hermetically 

 sealed. Cultivations were made by infecting sterilized nutritive liquids 

 in the cultivation apparatus with small quantities of this pleural effusion. 

 The cultivation liquids used were pork and beef broths which had not 

 been neutralized and neutral veal broth. All the attempted cultivations 

 were successful, and the organism which multiplied was of identical ap- 

 pearance in each — it was a diplococcus or figure eight in form, and had 

 a tendency to adhere in short chains and small clusters. 



This organism was carried through three cultivations, each apparatus 

 containing about half an ounce (lo cc ) of liquid. August 2 experi- 

 ment Xo. 3 was made by inoculating 2 pigs with the third cultivation 

 of this micrococcus. One of these had a hypodermic injection of 4 CC - 

 and the other of 10 cc - of the cultivation liquid. This was made on 

 the inner side of both thighs and with the latter also between the fore 

 legs. 



There was swelling at the point of inoculation within twenty-four 

 hours ; but no marked increase of temperature until August 7, when it 

 reached 105^° with one, and 104f ° with the other, with impaired appe- 

 tite, thirst, and shivering. Two days later the skin over the entire ab- 

 domen was wrinkled, flabby, and in places losing its epidermis. From 

 this time they improved in general symptoms until August 17, when 

 the one that received the larger quantity of virus and which had been 

 most severely affected was killed for examination. At this time there 

 was extensive desquamation of the epithelium over the abdomen ; the 

 swelling at the point of inoculation had softened and contained pus. 

 There was swelling of the lymphatic glands of the inguinal and mesen- 

 teric regions, petechias of the serous membranes, and slight peritoneal 

 effusion. 



Experiment j\ r o. 4. — Three pigs, Nos. 26, 27, and 28, were inoculated 

 June 9 with a cultivation liquid seeded from the virulent effusion of a 

 pig that had died from the result of inoculation with a very fatal virus 

 received from Illinois. This cultivation liquid contained only micro- 

 cocci, the appearance of which are very well shown in Plate XI, which 

 was reproduced from a photograph. 



