BUREAU OF ANIMAL INDUSTRY. 



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June l4, all had elevated temperatures varying from 104° to 105§° P., 

 increased thirst, tucked up abdomens, swelling at the points of inocula- 

 tion, rigors, and secluded themselves in their bedding. The appetite 

 was still fair. 



June 20, there was complete loss of appetite, emaciation, and profuse 

 diarrhea. 



June 29, No. 27 died, and autopsy revealed congestion of intestines, 

 hepatization of right lung, with abundant effusion in the pleural, peri- 

 cardial, and peritoneal cavities. Inoculations with this effusion caused 

 death of another pig July 8, after showing the well-known symptoms of 

 swine plague. 



July 3, No. 28 was found in a dying condition and was destroyed, in 

 order to get fresh material for examination and for inoculation experi- 

 ments. 



July G, No. 2G died in convulsions after having presented the charac- 

 teristic symptoms of the various stages of swine plague. 



The notable point in this experiment is the virulence of the cultivated 

 virus. This virus was a pure cultivation of micrococci and produced fatal 

 results in every case. The results of our inoculation experiments with 

 cultivated micrococci have heretofore been more or less unsatisfactory, 

 because, while the symptoms were those of swine plague, the disease 

 produced did not correspond in its malignancy to the swine plague which 

 so frequently decimates the herds of the West. In this case, however, 

 the disease developing as a result of inoculation had all the malignancy 

 of the most severe outbreaks which I have ever witnessed, and in 

 subsequent experiments with virus obtained from these animals this 

 fatal type has been retained and every animal inoculated has suc- 

 cumbed. 



On July 3, pig No. 34 was inoculated with mixed pleural and perito- 

 neal effusion obtained from No. 28, which was killed that day in the 

 last stages of swine plague, produced by inoculation with cultivated 

 virus as detailed above. July 15, the temperature was 104° F., and 

 there were periods of shivering. From this time the progress of the 

 attack was rapid; there was a red blush of the skin over the abdomen, 

 diarrhea, loss of appetite, prostration, and tendency to hide in the litter. 

 July 18, it was very much debilitated, the breathing was rapid, and it 

 was scarcely able to walk. It would undoubtedly have died in a few 

 hours. It was killed for examination and for pure vims. 



Autopsy showed the lungs to be covered on pleural surface with pete- 

 chias, but there was no hepatization. The inguinal and mesenteric 

 glands were greatly enlarged and congested ; the small intestines in- 

 flamed ; the caecum was the seat of three large ulcerous patches, 1 

 to 2 inches in diameter, and several of smaller size. These were black 

 on the surface and on sections the tissue appeared dense, fibrous, and 

 pale. The ileo-caecal valve was completely covered with such an ulcer, 

 and the mucous membrane of the stomach was much congested. 



Thoroughly sterilized vacuum tubes were filled from the jugular vein, 

 from the right ventricle, and with the pericardial and peritoneal effu- 

 sion, each of which were abundant. At the time of the autopsy small 

 quantities of each of these effusions and of the blood were dried on 

 cover-glasses for examination in the laboratory. 



The tubes of peritoneal fluid when opened emitted a very disagree- 

 able odor of putrefaction. Stained cover-glass preparations showed that 

 it contained both micrococci and rods. Cultures of the same contained 

 micrococci, a bacillus with pointed ends, probably the Bacillus butyricus, 



