NEW MYXOPHYCEAH VLIOM i'OKTO RICO 



19 



the subgelatinons tegument being secreted on the outside of the 

 wall, and is colorless from the beginning of germination to ma- 

 turity. On the contrary, the resting spores of A. nigroviolacea 

 on germination secrete the gelatinous matrix, or tegument, on 

 the inside of the wall, which moves outward from the protoplast, 

 becomes thinner and apparently modified, and loses much of its 

 color. The new tegument remains faintly colored throughout 

 the life cycle. The modification of the spore wall does not seem 

 quite so profound as in the case of ^1. fjifjos, A. vuKjuifica, etc., 

 of this paper, in which the original morphological character of 

 the wall is entirely lost. 



Anacystis nigroviolacea sp. nov. 



Plate 3, figure 34 



Colonies spherical to irregular, 25-40 pi (rarely up to 80 jj) 

 diam., free; cells 8-16 (rarely up to 64) in a colony, spherical to 

 somewhat irregular, 4-5 |j diam, pale aeruginous, considerably 

 obscured by the colored tegument, not closely crowded; resting 

 spores of the same shape as the vegetative cells, 7-7.8 p diam., 

 with firm, smooth, dark-violet wall; tegument subgelatinons, firm 

 and dark violet on the outside, softer and less colored toward the 

 center of the colony. 



Growing among other algae on rocks about ten kilometers 

 north of Utuado, no. 1553, type; on limestone at Hato Arriba, 

 Arecibo, no. 1399 d ; between Arecibo and Utuado, no. 1476 a ; on 

 rock in Jayuya, no. 1770 c. 



Anacystis nigroviolacea is apparently closely related to Gloe- 

 ocapsa violaced. No statements are made in any of the de- 

 scriptions, as far as I can ascertain, regarding the life history of 

 that species, and in the absence of authentic material it is not 

 possible at present to state the relationship. A. nigroviolacea 

 has a definite life cycle producing the resting spores with modi- 

 fied walls characteristic of the genus as understood and outlined 

 here. For a discussion of the relation of A. nigroviolacea to A. 

 nigropurpurea of this paper see under that species. 



Anacystis cylindracea sp. nov. 



Plate 3, figure 35 



Colonies spherical, up to 125 \i diam., free ; cells up to 64 in 

 a colonj^, widely separated, cylindrical with rounded ends, bright 



