23 



of disease were present as in the case of Peiiicillium sp. Some of 

 the bees ran around at a great speed while others showed signs of 

 great drowsiness. June 31. Several of the bees showed the typical 

 staggering walk, now falling on one side, now on the other. July 3. 

 Ten bees dead with more or less swollen abdomens. July 5. All 

 of them dead. 



The contents of ventriculus and rectum were plated and the 

 fungus was recovered from both. 



Series II. 



July 23. 12 bees were nsed. O.i gram fnngoits material was 

 mixed in 10 c. c. honey. July 24. No sure signs of disease could 

 be detected. July 25. One dead; several wild and intoxicated. 

 July 26. One more dead; most of the rest drowsy. July 27. Five 

 dead, more or less swollen. July 30. All hut one dead. 



Penicillium condiianeum Westling. 



July 23. 12 bees were caged. O.i gram of fungous material was 

 mixed in 10 c. c. honey. July 24. Several sick having staggering 

 walk and glossy abdomens. July 25. Eight dead. A few dying 

 lying on the back unable to rise; other crawling around on the 

 side. July 26., All dead. 



The bees fed on Penicillium conditaneum had the abdomens but 

 slightly sw ollen and considerably less than those fed on Penicillium 

 sp. and Mucor Mucedo (see below). The toxic substance in this 

 fungus acts verv quickly as may be seen from the data above. It 

 is inferred that the bees are killed already before any considerable 

 quantity of food is taken in, which would account for the insigni- 

 licant swelling of the abdomen. 



Examination of the intestinal träet showed great masses of spo- 

 res particularly in the rectum. Some of the spores had germinated 

 producing germtubes and in some cases branched mycelium. 



Mucor Mucedo. 



Series I. 



July 12. 15 bees used. 0.3 grams fungous material were mixed 

 in 5 c. c. honey. July 14. None of the bees dead, but nearly all 

 sick. Some of them were sitting in the bottom of the cage drowsy 

 and dull, other were running around at a great pace. When try- 



