22 



and scratched the tongue and the abdomen. June 28. The symp- 

 toms of disease were more striking. They were uneasy and rest- 

 less; pnshed the abdomen against the bottom of the cage, raised 

 themselves on their hind legs and tried in vain to fly. The ab- 

 domen had taken on a darker color 2 . 



June 29. Great drowsiness. No sound when the cage was given 

 a knock. They did not pay any attention to pure honey that was 

 given them. June 31. New honey and fungous material was given 

 in the same quantities as before. They ate with good appetite, 

 became wild and ran around as intoxicated. Some had in the 

 evening distinctly swollen abdomens. July I. Two dead, with 

 swollen. black and glossy abdomens. July 3. Eleven bees dead; 

 the rest very drowsy and hardly able to move. July i. All dead. 



When dissected ventriculus and particularly rectum were fonnd 

 to be dilated and swollen. The most swollen could hardly be touch- 

 ed without spurting out a green yellowish liquid, which received 

 its color mainly from the masses of spores of Penicillium sp. pre- 

 sent in the fluid. The contents of ventriculus and rectum were 

 plated and the fungus was recovered from both. 



Series II. 



July 23. 12 bees were caged. Oj gram fungous material was 

 mixed in 10 e. c. honey. July 2 i. One bee sick having swollen 

 abdomen. July None of the bees dead, but one showed the 

 symptoms of the disease very plainly. It hopped along trying to 

 lly but the abdomen was too heavy and swollen. It sometimes 

 flapped the wings vigorously. The rest were rather lively. July 26. 

 One dead; several had swollen abdomens and staggering walk. 

 July 27. Seven of the bees dead, all of them swollen. The rest 

 apparently sick. July 28. One more dead; the rest hopping 

 around with great difficulty. July 30. All dead. 



Penicillium sioloniferum Thom. 



Series I. 



June 27. 18 bees were caged. 0.5 grams of spores and myce- 

 lium were mixed in 10 c. c. honey. June 28. The same symptoms 



2 ) The abdomen becomes in many instances entirely shiny and glossy when the 

 disease has advanced for some time. This is snrely due to the fact that the bands 

 of down liave been worn off by the incessant scratching of the abdomen with the 

 hind legs. 



