August, 1911.] 



135 



Live Stock. 



The following schedule shows the result in a tabulated form :— 

 Each of the above tests was done in two animals, but I only give one typical 

 chart of each reaction : — 





Doses of 

 serum. 



8 c.c. 

 reaction. 



10 c.c. 

 reaction. 



12 c,c. 

 reaction. 



15 c.c. 

 reaction. 



Serum prepared 

 by Uie injection 

 of V. B. 



Serum prepared 

 by the injection 

 of peritoneal 

 fluid. 



With V. G. 

 Plains. 



With P. F. 

 Plains. 



Both mild 

 reaction. 



One death, 

 one severe 

 reaction. 



Both very 

 mild re- 

 action. 



Both severe 

 reaction. 



Both very 

 mild re- 

 action. 

 One death, 

 one severe 

 reaction. 



No reaction. 



Both mild 

 reaction. 



Result. — Virulent blood is a 10 c.c. serum. 

 / Peritoneal fluid is a 15 c.c. serum. 



(a) Therefore serum prepared from plains animals bv the hyperimmunizing with 

 virulent blood is 33 - 8 per cent, better than that prepared by hyperimmunizing with 

 injections of peritoneal fluid. 





Doses of 

 serum. 



4 c.c. 

 reaction. 



6 c.c. 

 reaction. 



8. c.c. 

 reaction. 



10 c.c. 

 reaction, 



Serum prepared 

 by the injection 

 of V. B. 



Serum prepared 

 by the injection 

 of peritonea] 

 fluid. 



With V. B. 

 Hill. 



With P. F. 

 Hill. 



Slight re- 

 action. 



Death. 



Both 

 slight re- 

 action. 

 Both dead. 



Both very 

 slight re- 

 action. 

 One death, 

 one severe 

 reactitfn. 



Very mild 

 reaction. 



Very mild 

 reaction. 



Result. — "Virulent blood is a 5 c.c. serum. 



Peritoneal fluid is a 10 c.c. serum. 



(b) Therefore serum prepared from hill animals by the hyperimmunizing with 

 virulent blood is 50 per cent, better than that prepared by hyperimmunizing with 

 injections of peritoneal fluid. 



(c) Serum from plains and hills in equal parts and made by blood injections is 

 depreciated 42 per cent, by the addition of an equal part of serum made by the 

 method of hyperimmunizing with peritoneal fluid. 



Although the above results show a 

 depreciation in the value of the serum, 

 it in no way detracts from the practical 

 utility of the method, in fact, it enorm- 

 ously increases the inoculable material, 

 and I may say that, but for its appli- 

 cation the laboratory would this year 

 have been unable to turn out anything 

 like the quantity of serum it has done 

 on account of the shortage in the supply 

 of hill animals for control purposes. It 

 will be seen that the power of serum pro- 

 duced from plains animals by the 

 inoculation of peritoneal fluid is less 

 deteriorated in comparison to the blood 

 method in similar animals than is the 

 serum frcm hill animals prepared in the 

 same way and compared with the blood 

 method in hill animals. This accentuates 

 the conclusion that the serum of 

 rinderpest is an anti-toxic material 

 and not entirely anti-bacterial. It has 

 already been noted thatjplains are much 

 less susceptible than hills, and yet from 



a material which is evidently more toxic 

 than blood they produce a better serum, 

 in comparison, than do the more 

 susceptible hill cattle. 



It is generally recognized that the more 

 susceptible an animal is to bacterial 

 influence, the less susceptible it is to the 

 toxin of that bacteria, and vice versa. 

 This appears to me strong evidence in 

 favour of the above conclusion that 

 anti-rinderpest serum is an anti-toxin. 

 Experiments have been commenced 

 on these lines, and the results of the 

 investigation will form the subject- 

 matter of another paper. 



Conclusions. — That the method of 

 employing peritoneal washings to aug- 

 ment the amount of inoculable virulent 

 material is a good one. 



(2) It produces anti-rinderpest serum 

 of a high value, but less potent than 

 that produced by blood inoculation. 



