6 J. W. W. STEPHENS—METHODS FOR DETECTING SPOROZOITS 
PARASITES THAT MAY BE MET WITH DURING DISSECTION. 
1. Trematodes—encysted in the thorax, also free in the stomach. 
2. Nematodes—in the thorax or abdominal cavity. In the thoracic muscles, 
filarial larvee may also be found. 
3. Sporozoa—(a) masses of sausage-shaped bodies about the salivary glands ; 
() sporoeysts containing 8 spores—these occur in large numbers replacing the 
yelk of the eggs (Fig. 5); (¢) gregarines, free in the stomach or encysted in 
the Malpighian tubes. 
Oc lo-Spoveqoe elk of ovum Achlace h Sperozoa im o wbrowt th 
by Plasheroroa Salivary Clavds 
Tagellale boares tn Aind- gu (7 
Fig. 5.—Protozoa other than the malarial parasite found in Anophelines. 
4, Flagellata—in large numbers in the gut. They are Crithidia-forms. 
5. Micro-organisms and Nosema (?) in the diverticula. 
6. Ecto-parasites—The reddish larval stages of water-mites (Hydrachnidae) 
are common. ‘The adult stages of these are unknown. 
INFECTION OF ANOPHELINES. 
Our information on this subject is deplorably inadequate. A systematic 
examination of suspected species at all seasons of the year would certainly yield 
results of considerable practical value. The following are examples of some of 
the results already obtained (Stephens and Christophers) :— 
ee Species of Percentage infected 
ocality. : : ; 
y Anopheline. with sporozoits. 
Lagos Town ... ae Pyretophorus costalis ... 3 
Olokemeji (S. Nigeria) Myzomyia funesta... 50 
Aro (S. Nigeria) ae Myzomyia funesta... 25 
DO = 14 Myzomyia culicifacies... 4°6 
Mian Mir (Punjab) ... be), y oe 
i] Myzomyia rossi wee 0) 
7 Myzomyla culicifacies... 8°6 
Ennur (Madras) soMy ih yy 
U Myzomyia rossii an 0 
Nor should the Culicines be neglected in this respect. An investigation of 
the commoner house-frequenting species might yield important information, 
