4 J. W. W. STEPHENS—METHODS FOR DETECTING SPOROZOITS 
(e.) Sporozoits are seen in such a fresh preparation in hundreds as thin, rather 
glistening, curved rods. The diaphragm should be properly adjusted (fig. 3). 
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Fig. 3.—Sporozoits in the salivary gland. 
Staining of Sporozoits. 
(a.) Smear off the cover-glass. 
(b.) Dry as rapidly as possible (over flame), 
(c.) Fix in alcohol for a few minutes—5 is ample. 
(d.) Stain in Romanowsky or Leishman, &c.—10 minutes or longer. 
Compare carefully sporozoits from quartan, simple tertian and malignant 
tertian cases. Also examine if there is any evidence of 3d, Q and indifferent 
sporozoits, 
Dissection of the mid-gut (stomach). - 
N.B—Keep the mosquitos, if caught in huts, for two to three days till the 
blood is all digested, as it is otherwise almost impossible to see zygotes. 
(a.) Place the mosquito (either a fresh one or the one from which the glands 
have been extracted) on cts back in a drop of clean salt solution. With a needle, 
nick or tear the chitinous body wall on each side as near the “tail” as possible. 
Place the left needle on the thorax and with the right needle on the extreme tip 
make a gentle traction as before. 
(}.) If carefully done, all the viscera will be pulled out, viz., oesophagus and 
diverticula, mid-gut, Malpighian tubes (5), hind-gut, ovaries (2), &c. When 
the tension is relieved the mid-gut assumes a flask-shaped saccular appearance. 
(c.) Cut the mid-gut off just below the Malpighian tubes. Also cut through 
the upper end of the oesophagus to which may be still attached the diverticula--- 
recognisable by the gas bubbles (COz2) they contain. 
(d.) Remove everything from the slide except the mid-gut (stomach). Adda 
clean drop of salt solution. Drop on a cover-glass and press gently, so as to 
flatten out the stomach. Hxamine with a 23-inch lens, taking care to adjust the 
diaphragm properly. 
