AND ZYGOTES IN MOSQUITOS INFECTED WITH MALARIA. 3 
De not flood the slide with salt solution as the glands when extracted may float 
away and be lost. Remember that the glands lie just at the point of origin of 
the first pair of legs. 
(b.) Place the slide upon a piece of white paper, as you are dissecting a dark 
object. Take a needle (in a holder if convenient) in each hand, holding them 
nearly horizontal. Place the left needle on the thorax so as to steady it and the 
right needle at the back of the head (fig. 1), and now make a series of gentle, slow, 
“coaxing” pulls on the head with the right needle, taking about 4-4} a minute 
over it. 
(c.) If this has been done carefully (and anyone with the least delicacy of 
touch can succeed 99 times out of 100), a bit of white tissue is now seen attached 
to the head. This contains the glands. 
(d.) Examine under about 4-inch lens, remembering nearly to close the diaphragm 
as an unstained object is being examined. The glands are glistening, finger-like 
and a little twisted, and project from the end of the head, or possibly only their 
tips may be seen in the midst of some grey muscle or “ fat body.” 
(e.) Separation from head :—Put the /eft needle on the head and with the right 
sut or tear off the white tissue containing the glands. (N.B.— Take care that by 
this time the salt solution has not dried or that the glands have not stuck to the 
needle, ) 
Examination of the Glands. 
(a.) The glands are generally quite free when dissected in this way, but even 
if still attached to a bit of tissue it is quite unnecessary to separate them any 
further. It is not necessary that all six lobes should be present, three may be 
considered sufficient (fig. 2). Note the exact position on the slide with a low 
power. 
Duel of «Mer she, 
SS Clal land 
Sa 7 
Common = 
duel 
dug 
Onlya acenav 
Fig. 2.—The salivary glands of one side. 
(6.) If the salt solution has become turbid carefully draw the glands into a 
fresh drop of solution on the slide, remembering that apparent right and left under 
the microscope are actual left and right on the slide. 
(c.) Drop a cover-glass over them. 
(d.) Examine with a } or? inch lens. An oil immersion is unnecessary for 
detection of sporozoits as they are in length about twice the diameter of a red 
cell, 
19127 A 
