2 J. W. W. STEPHENS—METHODS FOR DETECTING SPOROZOITS 
careful examination with a pocket-lens should enable you to state almost with 
certainty whether or no all the Anophelines you have caught are of the same 
species. Retain say a dozen, including some males, for examination by an 
expert. Kall them in the test tube by placing a drop of chloroform on the plug. 
Drop them on to a layer of very loosely fluffed cotton-wool in a pill-box or 
match-box. Place a very thin layer of loosely fluffed cotton-wool over them to 
prevent shaking. If obtainable, add one drop of creosote to the inside of the 
lid of each box to prevent mould, or melt a little naphthaline and pour it into the 
lid. 
Put a label outside the box stating when and where caught and name of sender. 
Dispatch home at once. 
DIssecTION OF A Mosquito. 
Killing.—This is most easily done by concussion, 7c. rapping the tube, with 
the mosquito at the end, smartly against the knee. (N.B.—This must on no 
account be done if the mosquitos are required for identification, as most of the 
scales are knocked off in the process). 
Trimming.—Put the mosquito on a slide, and while holding one wing pull off 
with a needle or forceps the legs and remaining wing. ‘Take care not to pull 
off the proboscis by mistake. 
Dissection of Salivary Glands. 
(a.) Put the trimmed mosquito into a drop of salt solution (1 per cent, roughly) 
on a slide so that it lies on its right side with its proboscis towards you (fig. 1). 
Fig. 1.— Diagram to illustrate the method of extracting the salivary glands of a mosquito.* 
* his drawing has been kindly made for me by Mr. H, F, Carter, Assistant Entomologist, 
Liverpool School of Tropical Medicine, 
