METHODS FOR DETECTING SPOROZOITS AND ZYGOTES 
IN -_MOSQUITOS INFECTED WITH MALARIA. 
BY. W, Wao LEPHENS, M.D... Cantras., D.P.H. 
Walter Myers Lecturer in Tropical Medicine, Liverpool School of Tropical 
Medicine. 
CAPTURE AND PRELIMINARY PREPARATION OF MOoSsQuITOsS. 
Where to find infected Anophelines. 
(a.) Select a village or any small colle¢tion of native huts where you know, 
either from a blood examination or from spleen palpation, that the children are 
infected with malaria. 
(b.) Anophelines and also Culicines will be found in the huts, near the fire- 
place, behind curtains, in cupboards, behind clothing, in old boots, collections of 
bottles, lumber, rubbish of all sorts, and on the thatch of the huts hanging to the 
sooty straw and cobwebs, often in hundreds, If none can be found, stir the 
thatch with a stick, as Anophelines frequently lie deeply hidden. [Examine also 
out-houses, cowhouses, piles of dried grass in barns, and the outside of mosquito 
nets before the sun is well up. 
(c.) In short drains or under bridges they may be sometimes caught in 
hundreds. 
(d.) Or finally, “traps” may be set. These are made out of roomy biscuit 
tins (lined with felt), with the lid ajar. Mosquitos retreat into these as the sun 
rises, 
Method of Capture. 
Place a test tube or specimen tube s/owly over the Anopheline, and then close 
it by slipping a plug of cotton wool into the mouth, 
It will save a good deal of time if fifty mosquitos are required, to bring fifty 
test tubes instead of transferring each one when caught to a bottle. Also the 
trouble of getting them out of the bottle is avoided. But in case the supply of 
tubes is insufficient they should be transferred, as caught, into any dry bottle, 
such as a whisky bottle. There must be no obvious moisture in the bottle, as if 
there is, the mosquito is caught by its wings and soon dies. 
Identification of Species. 
There is still very little accurate knowledge as to which species actually do 
transmit malaria, and also as to which of these are the most concerned. It is 
essential then to identify, or to get identified, the Anopheline you dissect. A 
(19127—2.) Wt. P 3528—16. 1000, 4/11. D&S. 
