28o 



s. isilll : 



that the structures that there formerly taken for intracellular gregarincs 

 are merely certain cell-inclusions. 



ci ci 



ei 



A portion of a cross-section through the intestinal epithelium of 

 meal-worm, showing cell-inclusions {ci) and stipes of intracellular 

 Grigarina polymorph». //, cell nucleus. (X500). 



As to the method employed by me, meal-worms were cut at both 

 ends ; then, the whole digestive tract was pulled out and fixed with 

 SCHAUDINN's sublimate-alcohol or with acetic-sublimate. Sections were 

 stained with iron-haematoxylin and counterstained with orange-G. 



The epithelial cells of the intestine commonly contain numerous 

 round or ovoid bodies besides the nucleus. It is certain that some of 

 them are metaplasmic products due to secretion or some other cause, 

 but many others are undoubtedly young gregarines. 



The metaplasmic cell-inclusions are ovoid or elliptical in shape, 

 and are very small, their major axis measuring only 2-10 fi. They 

 may occur at any position in the epithelial cells. Usually a single 

 inclusion is found within an epithelial cell ; and in most cases it con- 

 tains one or more nucleus-like granules, deeply stainable with iron- 

 haematoxylin. The remaining part of the inclusion takes an orange 

 color. The small bodies with one or more " Kerne," which BERNDT 1 

 has found in the intestinal epithelium of the larva of Tcncbrio moritor 



1) Berndt, A., '02. — Beitrag zur Kenntniss der im Darme der Lirve von Tenebrio 

 morilor lebenden Gregarinen : Arch. f. Protisten k., 1. 



