282 



S. ISIIII : 



The larger intracellular individuals lie nearer to the intestinal lumen 

 than to epithelial base, with their longitudinal axis disposed verticali)' 

 to the surface. In length they are about equal to one-third or even 

 one-half of the whole epithelial thickness, while their lateral expansion, 

 including the clear space round their body, covers an extent of from 

 four to eight host cells. 



The clear space just mentioned is ovoid or subsphcrical in shape. 

 In the fresh state, that space is found to be filled with some fluid 

 apparently secreted by the surrounding cell substance; the fluid may 

 possibly serve as nutriment to the gregarine contained in it. The space is 

 generally widest along the sides of the gregarine and narrows towards 

 both ends. The posterior end of the gregarine is often in direct contact 

 with the cytoplasm, all other parts lying free in the clear space. 



The intracellular gregarines are yet very poor in entocytic granules, 

 and, further, the granules present are still very fine. The entocyte is 

 generally less dense in the protomerite than in the deutomerite. Some- 

 times there exists a clear region devoid of any granule in the anterior 

 parts of the protomerite and behind the septum. The nucleus is com- 

 paratively large, its diameter being equal to about half the breadth of 

 the body at the region, and is lodged in the centre of the deutomerite. 

 The karyosome is always single and is very large. It stains most 

 intensely with iron-haematoxylin, while the nuclear sap remains feebly 

 colored. It is so strongly retentive of nuclear stains that it remains 

 colored even when the chromatin of host cells is made to lose the 

 stain, — a fact which affords great help in discovering the gregarines 

 in the epithelium. 



In most cases the protomerite is directed towards the lumen of the 

 intestine, — a remarkable fact clearly demonstrable in our gregarines. 

 One might think of three different ways to account for this phenomenon : 

 (a) the sporozoite may have entered the epithelium with its posterior 

 end foremost and developed the protomerite at the anterior end ; (b) 

 contrarily, the sporozoite may have entered the epithelium with its 



