i9o5.] Inoculation of Leguminous Plants. 



643 



The illustrations (Figures 1 and 2) show the wire cage, pro- 

 tected by waterproof roller-blinds, in which Professor Hiltner 

 conducts these trial experiments. 



Dr. Moore's cultures were sent out absorbed on cotton-wool, 

 covered with tinfoil, and accompanied by two kinds of salts 

 to be used with water in making fresh cultures. 



The directions given by Dr. Moore were closely followed in 

 most cases, but it should bs mentioned that the cloudiness, which 

 is said to be produced in the solution as a result of the growth 

 of the organism, was not always observed. It was also noted 

 that the liquid contained bacteriological impurities (such as 

 Oidiuin lactis) in great quantity. The manipulation of these 

 cultures does not seem to have presented any serious practical 

 difficulties ; it is, however, difficult on a farm to keep the neces- 



Fig. 2.— Another View of Dr. Hiltner's Experiment House. 



sarily large volumes of liquid at the temperature necessary for 

 the growth of the organisms, and unless this is done no satis- 

 factory result can be expected, if, as is claimed, the organism 

 which grows in these solutions is to be capable of infecting the 

 plant. 



The inoculation of seed or soil is easy and rapid with either 

 kind of culture. Seeds, for instance, can be placed in butter 



FFF2 



