646 Inoculation of Leguminous Plants. [feb., 



The early formation of nodules was about in the same pro- 

 portion, Moore's cultures being behind Hiltner's in this respect- 



The air-dried peas per pot weighed in grams (Figure 3) : — 



Peas : Sand, no inoculation (c) — 



Seed ... ... ... ... 1:51 grams-* • „, - 



Pods and stems r8o „ } = 3 V. grams. 



Peas : Sand with ammonium nitrate {d) — 



Seed • 6-5 grams! _ fi 



Pods and stems 8 66 „ / ~ 5 " 



Peas : Sand inoculated with Hiltner's culture {a) — 



Seed 9-31 grams j = 



Pods and stems ... ... 5*60 ,, J" ^ y " 



Peas : Sand inoculated with Moore's culture (/) — 



Seed 5-98 grams, = 



Pods and stems ... ... 4*52 ,, j 3 " 



Beans in sterile sand did not produce such good results, and 

 though a few nodules were formed on those inoculated with 

 Hiltner's culture, none were formed on the roots of those inocu- 

 lated with Moore's culture. 



Woburn. — Hiltner's cultures for peas, beans, tares, and red 

 clover ; and Moore's cultures for Melilotus and soy bean : soil 

 light sandy loam, sterilised by heating for six hours in a copper 

 with water raised to boiling temperature, soil then drained, dried 

 and sieved, and filled into earthenware pots. 



The seed was inoculated, and duplicate pots compared with 

 duplicate uninoculated pots. The seed was kept in the inoculat- 

 ing preparation for forty-five minutes in the dark. 



In the pot experiments, twelve seeds were sown per pot for all 

 the crops except Melilotus, of which twenty-two seeds per pot 

 were planted. The dates of sowing were : — Peas and beans, 

 April 3rd ; tares and clover, April 17th ; soy bean, April 22nd; 

 Melilotus, April 29th. 



By May 25th, peas and beans looked well, and at first the 

 treated seed appeared better, the foliage having a darker colour ; 

 this difference, however, soon passed cff. On June 8th, the 

 plants were thinned to six per pot. The superfluous bean plants 

 thus removed were washed and photographed. The photographs 

 showed, however, practically no difference between the treated 

 and untreated seed, and it was remarkable that plants in steril- 

 ised soil, and showing no nodules on the roots, had made such 

 good growth. A similar result was obtained with tares. 



