We have consequently thus far seen that Cinchona leaves 

 contain alcaloid, which alcaloid disappears inside of 12 

 hours from the leaf attachée! to the stem, while in eut lea- 

 ves it remains even after several weeks. 



Now the question présents itself, wether empty leaves 

 are able to form alcaloid again, inside of a short period. 



That leaves connected with the stem are able to do so 

 is proved by the next experiments: 



Sept. 20. 6 a. m Sept, 20 6. p. m. 



294 empty fnll 



295 „ „ 



296 • 

 297 

 298 



299 traces „ 

 300 



301 • „ 

 302 



311 traces „ 

 In thèse cases the possibility remains that the origïnal- 

 ly empty leaves got the alcaloid lateron found in them 

 from the alcaloid présent in the bark. 



It was therefore necessary to demonstrate that eut emp- 

 ty leaves, were able to form alcaloid if brought under fa- 

 vorable conditions. 



The next experiments show that such can be done. 

 Half of leaf examined Correspond! ng half in H 2 0 





JllllJ 11. 



+ 1/4 % N H 4 until July 17 



No. 141 



empty 



fllll 



142 



empty 



full 



145 



empty 



full 





July 17. 



July 24. 



172 



empty 



full 



173 



empty 



full 



