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Scientific Proceedings (25) 



increase in the urine volume for the entire experimental time rang- 

 ing from 3.1 per cent, for 7 days after 4^ hours anesthesia to 

 20.7 per cent, for 7 days after 2 hours anesthesia. In one experi- 

 ment narcosis for 1 hour caused a diuresis which was persistent 

 throughout the remaining 10 days of the experiment during which 

 time the urine output was increased a total of 710 c.c. or 18.4 

 per cent. 



Anesthesia caused the animals to lose body weight, the per- 

 centage loss during the 24 hours following anesthesia varying 

 from 1 per cent, to 3 per cent. The loss of weight following the 

 anesthesia of a dog for periods of 1, 2 and 3 hours on three suc- 

 cessive days was 6.5 per cent. 



In all except two experiments the reaction of the fractions of 

 urine first voided after anesthesia was amphoteric, alkalinity gener- 

 ally predominating. In the two experiments noted the urine was 

 acid both before and after anesthesia. The specific gravity of the 

 first urine passed after ether narcosis generally ranged from 1024 

 to 1042 as compared with a normal specific gravity ranging from 

 1015 to 1019. 



13 (269) 



The relative value of antitoxin and other curative substances 

 in antidiphtheric serum. 



By EDNA STEINHAEDT and EDWIN J. BANZHAF. 



[From the Research Laboratory of the Department of Health, 

 New York City.~\ 



Cruveilhier, in a paper in the Annates de V Institut Pasteur 

 (XVIII, p. 249), quotes Roux, Marfan, Martin and Momont as 

 finding that the dose of antidiphtheric serum which is most effica- 

 cious is not always the one that contains the greatest number of 

 antitoxic units. They assert that in the serum, there are, besides 

 antitoxin, other substances whose therapeutic value is ignored in 

 the present measurement of antitoxic units. 



Cruveilhier carried out a series of experiments, in animals 

 infected with diphtheria bacilli, and compared sera of different anti- 

 toxic strengths from several horses as to their value preventively 

 and curatively. His results apparently showed that it was the 



