n6 



Scientific Proceedings (29). 



Flow did not continue. 5.00. Dog allowed to smell meat. 

 About 10 c.c. clear juice with a few fine floculi. 5.15. Dog 

 allowed to eat meat. Rate of secretion apparently not increased. 



The animal stood whole procedure well and showed no ill 

 effects afterwards. Died Nov. 13, 1907. 



Dog 2. Operation. Simple gastric fistula. Wound healed 

 per primum. 



April 6, 1908. Dog fasted for past thirty-six hours. 11. 15 

 A. M. Stomach catheterized ; 0.9 c.c. of fluid removed. 11.45. 

 Left external jugular vein exposed under light chloroform anes- 

 thesia and 5 c.c. of 0.7 per cent. NaCl injected intravenously. 

 11.50. Stomach catheterized; 3.3 c.c. of fluid removed. Dog 

 allowed to run about. Not uncomfortable from the chloroform. 

 12 M. Stomach catheterized. No fluid removed. Showed and 

 allowed to smell raw meat for 10 minutes. 12.10 P. M. 

 Stomach catheterized; 4.3 c.c. of fluid removed. 12.15. Dog 

 given full meal. 



April 8, 1908. Dog fasted for past thirty-six hours. 2.45 

 P. M. Stomach catheterized ; 3 c.c. of fluid removed {specimen C). 

 3.00. Stomach flushed with tap water. 3.10. Left external 

 jugular vein exposed under light chloroform anesthesia and 8 c.c. 

 submaxillary extract injected intravenously. (The glandular ex- 

 tract was prepared as follows : The two submaxillary glands were 

 excised from a perfectly healthy dog under morphin-ether anes- 

 thesia, macerated with sterile sand in sterile 0.7 per cent. NaCl, 

 and filtered through cloth.) 3.35. Catheterized; 9 c.c. of fluid 

 removed {specimen B). 3.40. Showed and allowed to smell raw 

 meat for ten minutes. 3.50. Catheterized; 9 c.c. of fluid removed 

 {specimen A). 



The three specimens of gastric fluid thus obtained were 

 analyzed by one of us without knowledge of the foregoing pro- 

 cedure. The results of this analysis were as follows : 



April 8, 1908. Analysis of gastric juice from dog with gastric 

 fistula. I c.c. of the unfiltered juice was titrated with N/20 NaOH 

 for the total acidity (phenolthalein) and free HC1 (dimethyl- 

 amidoazobenzol). 



Specimen A. Total acidity = 1-95 c.c. N/20 NaOH 

 Free HQ = 1.30 c.c. " 



