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Scientific Proceedings (29). 



excreted during the first few days after transfusion. However, 

 the diuresis following transfusion of blood is very much less than 

 that produced by infusion of 0.9 per cent. NaCl solution. In the 

 case of the latter, the total volume infused appears in the urine of 

 the succeeding twenty four hours. Nevertheless, the diuretic 

 effect of blood transfusion is quite marked. 



The excretion of nitrogen in the urine was decidedly increased 

 by the transfusion of blood, the rise becoming more and more 

 pronounced with each successive transfusion. To cite one in- 

 stance : The average daily urinary nitrogen rose from 6.28 grams 

 in the preliminary period of nitrogen equilibrium to 10.23 grams 

 after the 4th blood transfusion. To what extent the different con- 

 stituents of the blood contribute to this result ; whether the increase 

 of nitrogen is derived from the blood (the serum or corpuscles), or 

 from the body proteins destroyed by any toxic action of the trans- 

 fused material, or whether the increase is caused by both (the most 

 probable thing) will be investigated later. 



"Blood counts" show that the number of erythrocytes per unit 

 of volume rises with each transfusion, which fact is due to the elim- 

 ination of water, the red cells being retained in the vascular sys- 

 tem a much longer time, in this manner causing a distinct poly- 

 cythemia. By a series of transfusions it was possible to increase 

 the number of erythrocytes from 7,272,000 to 13,512,000 in one 

 cmm. In this connection it is interesting to note that, after rising, 

 the " blood count " showed a decline which set in after a few days. 

 Then another transfusion was performed, and the number of red 

 cells increased still further. In this manner a continued and more 

 and more pronounced polycythemia was produced. The specific 

 gravity of the blood rose also with each transfusion. It is note- 

 worthy, also, that after a few transfusions the specific gravity was 

 relatively higher as compared with the number of red cells, show- 

 ing a greater concentration of the blood serum proper. The specific 

 gravity rose after a few transfusions from 1.066 to 1.103. 



No decided increase in the nitrogen output has been observed 

 after saline infusion. The daily urinary output of nitrogen from 

 the control dog increased from 5.29 grams in the preliminary 

 period of nitrogenous equilibrium to 5.91 grams after the ninth 

 saline infusion. 



