Scientific Proceedings. 



(199) 29 



restored spontaneously without washing with Ringer solution, if 

 the application of the magnesium solution was not renewed. After 

 the application for two or three hours of strongly hypertonic solu- 

 tions, the conductivity did not return usually for 24 hours or 

 longer, sometimes not even after washing with Ringer solution, as 

 was observed in experiments on the sciatic and on the superior cer- 

 vical ganglion. Thus far the experiences of the authors in this 

 connection indicate, however, that conductivity is finally restored 

 in all cases. 



Fourteenth meeting. 1 



Rockefeller Institute for Medical Research. December 20, ipoj. 

 President Wilson in the chair. 



10 (102). " The action of eosin upon tetanus-toxin and tet- 

 anus " : SIMON FLEXNER and HIDEYO NOGUCHI. 



Eosin and certain other anilin dyes have the power of destroy- 

 ing in vitro the hemolytic property of tetanus-toxin. 



Eosin, when used in sufficient quantity, destroys tetanospas- 

 min in vitro. 



Simultaneous injection of tetanus-toxin and eosin into rats 

 delays or prevents the appearance of the symptoms of tetanus. 

 When the symptoms appear they progress more slowly than in 

 control animals. 



Spores of tetanus-bacilli when introduced on threads into 

 rats, and followed immediately by an injection of eosin into the 

 same locality, do not produce tetanus. The treatment of animals 

 with eosin, after the first appearance of the tetanic symptoms fol- 

 lowing spore-infection, may prevent the further development of 

 the symptoms of tetanus. Eosin injections into the same locality 

 as the spore inoculations are the most effective, but injections into 

 other parts of the body delay or modify the tetanic process. 



Rats are more resistant to tetanus poison than guinea-pigs, and 

 hence are more easily protected by eosin from tetanus ; but in 

 guinea-pigs the fatal issue can be delayed by eosin. 



1 Science, 1906, xxiii, p. 109 ; American Medicine, 1906, xi, p. 105. 



