Scientific Proceedings. 



(227) 5 



cause, in addition to lesions of the aorta, degenerative changes in 

 the myocardium which are most marked after the fifth injection. 

 The majority of the animals (rabbits) which recover from the early 

 injections exhibit a fibrous myocarditis either focal or diffuse. 

 These proliferative changes are not analogous to those occasionally 

 produced experimentally by bacterial toxins, but resemble rather 

 those following obstruction of the coronary arteries. It is essen- 

 tially a process of repair following degeneration of muscle fibers. 

 The latter is due apparently to temporary ischemia of terminal vas- 

 cular territories at a time when the heart muscle exerts an increased 

 contractile effort necessary to overcome the greatly augmented 

 intra-vascular tension. Thus both nutritive and mechanical distur- 

 bances appear to play a part in its etiology. 



32 (124). " Stable and detachable agglutinogen of typhoid 

 bacilli": B. H. BUXTON and J. C. TORREY. 



By heating an emulsion of typhoid bacilli to 72 0 C. for half an 

 hour a detachable agglutinin may be separated from the bacilli. 

 This may be obtained in the filtrate on passage through a Berkefeld 

 filter. Rabbits, which have been inoculated on the one hand by 

 this filtrate and on the other by the heated bacilli, which have been 

 thoroughly washed, show specific differences in their serums, as re- 

 gards agglutination. The animal inoculated with the washed bacilli 

 or stable agglutinin, produces a serum which agglutinates normal 

 typhoid bacilli very slowly and with the formation of fine clumps. 

 In contrast to this, the filtrate containing only detachable aggluti- 

 nin gives rise to serum which clumps normal typhoid bacilli rapidly 

 and with the formation of large flocculi. 



Absorption experiments show, furthermore, that the s or stable 

 agglutinin and the d or detachable agglutinin are distinct in charac- 

 ter, for the heated and washed bacilli absorb nothing from the 

 filtrate serum, but absorb all the agglutinin for normal typhoid bacilli 

 from the bacillus serum. On the other hand the filtrate absorbs 

 nothing from the bacillus serum, but takes up all the agglutinin 

 from the filtrate serum. 



It has also been determined that the substance in typhoid bacilli 

 which gives rise to precipitins for filtrates of typhoid cultures is 

 split off from the bacilli, together with the detachable agglutinums. 



