70 (240) Society for Experimental Biology and Medicine. 



The acetone rhigolene mixture used in the preliminary purifi- 

 cation of the " protagon " group contains substances which possess 

 solubilities similar to those of Thudichum's kephalin and myelin, 

 both before and after precipitation with lead acetate. But these 

 substances have not yet been obtained in sufficient quantities for 

 satisfactory purification. They appear, however, to contain more 

 phosphorus (over 4 per cent.) and less nitrogen (about 1 per cent.) 

 than the analogue of sphingomyelin already considered, and these 

 characters are also in harmony with Thudichum's analyses of 

 kephalin, myelin and sphingomyelin. 



From the "lecithin" group, a cadmium chlorid compound 

 was obtained which, when purified with ether, acetone and alcohol, 

 resembled the cadmium chlorid compound of paramyelin (Thudi- 

 chum). The percentages of phosphorus and nitrogen in this com- 

 pound (150 A — CdCl 2 ), in a second sample purified but once with 

 ether and acetone (1 50 B — CdCl 2 ), and in Thudichum's paramyelin 

 from human and ox brains, are given in Table II. 

 r f £Still other compounds containing phosphorus, nitrogen and 

 fatty acids have been obtained from renal extracts, and appear 

 to be analogous to substances derived from the brain. But since 

 all these compounds require much further study before their con- 

 stitution, relations to each other and to substances originally 

 present in the tissues can be understood, it is believed that this 

 preliminary report need not be burdened with further, necessarily 

 incomplete analytical data. 



Table I. 



160 A. 



160 B. 



160 A — CdCl a 



160 B-CdCl 2 



Sphingomyelin. 1 



Apomyelin. 



P 2.493 



2.48 





3.690 



3-623 



3-24 



3-23 



N 2.869 



2.74 





2.896 



3.2II 



2.96 



3.OO 



C 63.570 







67.546 





65-37 



67.OI 



H 11.840 







12.445 





11.29 



11.35 



CdCl, — 







25.380 



25-362 



i6.6i to 26.59 



Table IL 













Paramyelin Cadmium Chlorid. 1 



150 A-CdCl, 





ISO B— CdCl a 

















Human Brain. 



Ox Brain. 



P 



4-348 





4.156 



4.78 



4-3>3 



N 



2.403 





2.219 



2.25 



2.029 



CdC! 2 25.990 





24.340 



24.95 



21.275 



Thudichum, L c, p. 170. « Thudichum, 1. c, p. 153. 



