8o (250) Society for Experimental Biology and Medicine. 



The results obtained were not uniform. Distinct cloudiness 

 on boiling appeared in the bile of one experiment both before and 

 after poisoning with amyl alcohol. On the other hand, in one of 

 the experiments, the bile remained perfectly clear on boiling before 

 and even after injection of amyl alcohol. After the administration of 

 small quantities of amyl alcohol, e. g., 5 c.c, there was no albumino- 

 cholia. Following the injection of 20-30 c.c, however, the bile 

 became distinctly cloudy on boiling after slight acidification with 

 acetic acid. In none of the experiments carried out as indicated 

 were more than traces of albumin found in the bile. One special 

 experiment, however, on a dog poisoned with ricin gave a different 

 result. The dog received in three days two subcutaneous injec- 

 tions of ricin, 1 mg. per kilo each time. He was found dead the 

 day after the last injection. The bile removed from the gall bladder 

 showed the presence of a considerable quantity of albumin. 



It seems probable, therefore, that the albuminocholia after 

 poisoning with ethyl or amyl alcohol, as observed in animals with 

 permanent fistulas, was due to irritation of the bladder and only 

 slightly to lesions in the liver. The question whether albumin 

 passes more readily into the bile than it does into the urine was 

 also studied. The result in every case showed considerable quan- 

 tities of albumin in the urine after poisoning with amyl alcohol. 



A few experiments on rabbits have also been undertaken. 

 Cantharidin or arsenic was injected subcutaneously until albuminuria 

 or hematuria was induced. A biliary fistula was then made and 

 bile collected. In none of these experiments was albumin found 

 in the bile. 



