22 



Scientific Proceedings (77). 



TABLE III. 



Variation in the Coagulation Time of Case I Following the Intravenous 

 Injection of about 300 C.c. of Freshly Drawn Human Phosphated Blood. 



Coagulation Time. 



Date. Minutes. Remarks. 



May 16 245 Normal coagulation time — 13 min. 



"20 11 A.M. About 300 c.c. of human phos- 



phated blood injected intra- 

 venously. 



12 Noon. 24 

 " 22 30 

 " 24 32 

 " 28 55 

 June 14 10 A.M. 200 



10:15 A.M. 8 c.c. of human serum 20 hours old 



injected intravenously. 



12 Noon. 38 



than before. Smaller amounts of normal plasma were required 

 to reduce the coagulation time of the recalcified plasma to normal 

 after serum injections than before. It was concluded that the 

 serum injection had altered the pro-thrombin content of the blood. 



These observations were made in 1910 in the Laboratory of 

 the Royal College of Physicians in Edinburgh during the tenure 

 of a Carnegie Research Fellowship. It was hoped that they 

 might be completed and extended before publication, but this 

 has not been possible. They are of interest in connection with a 

 recent report by Ottenberg 1 on the effect of citrated blood and of 

 citrate solution on the coagulation time in hereditary hemophilia. 



Conclusions. 



1. The intravenous injection of fresh human serum causes an 

 immediate shortening of the coagulation time of the blood in 

 cases of hereditary hemophilia. There is then a gradual lengthen- 

 ing of the time until it is considerably longer than before the 

 injection, and finally a return to the original level. 



2. The intravenous injection of fresh whole blood prevented 

 from coagulating by the addition of sodium phosphate caused a 

 similar immediate decrease, and was followed by a gradual increase 

 in the coagulation time during the subsequent eight days. 



3. An alteration of the pro-thrombin content of the plasma- 



1 Ottenberg, R., Proc. Soc. Exp. Biol, and Med., 1916, 13, 104. 



