Equine Sporotrichosis in Montana. 23 



was found to be the cause of the increased coagulability of the 

 blood after intravenous injections of fresh normal serum. 



15 ("93) 



Notes on the occurrence of equine sporotrichosis in Montana and 

 the "blastomycotic" form of Sporotrichum schencki-beurmanni. 



By K. F. Meyer. 



[From the George Williams Hooper Foundation for Medical Research, 

 University of California Medical School, San Francisco.] 



In 191 5 I 1 expressed the belief, based on very inadequate 

 material, that animal sporotrichosis is found also in Montana. 

 Quite recently, through the courtesy of Doctor DuFrene, of 

 Glendive, fresh pus collected from a case of equine sporotrichosis 

 was forwarded to me for diagnosis. Without the least difficulty 

 a typical Sporothrix schenki-beurmanni was isolated on Sabouraud 

 medium, and conclusive bacteriologic evidence was thereby ob- 

 tained that sporotrichosis exists endemically in Montana. 



As is customary in our studies on fungi, plain one per cent, 

 glucose agar was inoculated with the pus. The growth on this 

 medium remained perfectly white and thin, becoming thick, moist, 

 very stringy and inelastic in contrast to the typical well-pigmented 

 folded film observed on Sabouraud's agar. The culture did not 

 penetrate into the superficial layers of the agar, and was easily 

 emulsified. It grew well under anaerobic conditions, and pro- 

 duced a rapid septicaemia in rats and rabbits. On one per cent, 

 glucose agar and plain potato, this pleomorphism has remained 

 so far (three weeks and four transplants) constant, but on Sabour- 

 aud medium the typical growth invariably appeared in a short 

 time. 



Microscopically, such a culture consists of oblong, oval or 

 round, short, monilia-like mycelia with a well-marked double 

 membrane and refractile granules. Some round forms show repro- 

 duction by budding and aggregations in pairs or short chains. 

 Long mycelia with typical clusters of spores were always absent. 



1 Jour. A. M. A., 1915, LXV, p. 519. 



