Relation of Sewage Disposal to Pellagra. 



27 



monia were unsatisfactory because sufficiently large quantities 

 of a single fluid required for a test were not available. Mixed 

 water-clear sterile fluids obtained from poliomyelitis cases, were 

 used. The results range from 0.1 mgm. to 0.9 mgm. of ammonia 

 nitrogen per 100 c.c. 



For the determinations of creatinine and creatine the Folin 

 and Denis methods were employed. These methods have been 

 recently criticized by McCrudden and Sargent. 1 The results 

 nevertheless seem worth reporting in view of the creatinine and 

 creatine studies on blood with the same methods. About half 

 mgm. creatinine and from 0.3 to 0.7 of creatine were found in 

 fluids examined. 



Sugar was determined by means of the Lewis and Benedict 

 method. The findings in poliomyelitis are at a somewhat lower 

 level than that of the blood. 



Uric acid also was determined on mixed fluids, the results 

 indicating that measurable amounts are present in this fluid. 2 



Attempts to determine cholesterol by Bloor's 3 method showed 

 the presence of traces only. 



Average Chemical Findings in Spinal Fluids Expressed in Mgm. per 100 C.c. 



Number 

 of Cases. 





Total 

 Nitrogen. 



Non-protein 

 Nitrogen. 



Urea 

 Nitrogen. 



Creatinine. 



Creatine. 



Sugar 

 (Per Cent.). 



2 



Meningism. . . 



15 



13 





.36 





.07 



12 



Epidemic 

















cerebrospinal 



Si (24- 



23 (1 5-33 ) 



14 (I0-2I) 



•44 



.70 



Traces 





meningitis . . . 



120) 













8 



Tubercular 













Traces- 





meningitis . . 



30 (25-42) 



15 (13-17) 



9 (7-14) 



.48 



.56 



.06 



3 



Influenza 

















meningitis . . 



76 





6 









45 



Poliomyelitis . 



23 (16-34) 



15 (11-24) 



10 (5-20) 



■44 (-35- 



.38 (.19- 



.07 (.05- 













•51) 



•49) 



.09) 







Ammonia Nitrogen 

 = •58 



Uric Acid 



= Traces 

 5 



Cholesterol = Traces 



The figures in parenthesis indicate the lower and upper limits. 



1 McCrudden, F. H., and Sargent, C. S., J. Biol. Chem., XXVI, 527, 1916. 



2 Compare Fine, M. S., and Myers, V. C, Proceed. Soc. for Exp. Biol, and 

 Med., XIII, 126, 1916. 



» Bloor, W. R., J. Biol. Chem., XXIV, 227, 1916. 



