4 6 



Scientific Proceedings (78). 



trols. The proteose used in these experiments was prepared as 

 described from material obtained in cases of intestinal obstruction 

 or of closed intestinal loops. 



Dogs with isolated loops of small intestine show many evidences 

 of intoxication. A study of the total nitrogen elimination shows 

 a great rise above the normal base line minimum of this fasting 

 period. This means that this intoxication is associated with a 

 great destruction of body protein, and explains the high non- 

 protein nitrogen of the blood which was observed and reported 

 previously. 



Dogs injected with sublethal doses of proteose will show a 

 definite tolerance to subsequent injection, and will show much 

 less acute intoxication after the isolation of a closed intestinal 

 loop. Such immune or tolerant dogs show a much less pronounced 

 rise in the nitrogen elimination curve during proteose intoxication 

 of any type. This indicates that the tolerance or immunity to 

 proteose gives more protection for the body proteins against the 

 injury which these toxic proteoses inflict upon the body cells. 



Complete duodenal obstruction combined with a gastroje- 

 junostomy gives a chronic type of intestinal obstruction associated 

 with little vomiting, which is peculiarly suited to metabolism 

 study. Such duodenal obstructions show a definite and sustained 

 rise in the curve of nitrogen elimination above the normal base 

 line level. These dogs, too, are tolerant to injections of standard 

 toxic proteoses. 



Control ether anesthesia experiments show little if any rise 

 in the curve of nitrogen elimination. 



Control laparotomy experiments show a definite rise in the 

 curve of nitrogen elimination, but a rise which is small compared 

 with the rise noted in the intoxication of duodenal obstruction or 

 isolated intestinal loops. It is highly probable that the tissue 

 injury and disintegration associated with the wound reaction are 

 responsible for the general reaction. We may assume that protein 

 split products from the wound area are absorbed, and are respon- 

 sible for the general reaction observed. 



Metabolism studies on fasting dogs show that during the 

 intoxication accompanying the formation of a subcutaneous ab- 

 scess there is a marked increase in nitrogen elimination. This 



