Adrenalin. 



79 



4. No evidence has been obtained that after section of the 

 nerves of one adrenal, any compensatory increase in the rate of 

 liberation of epinephrin from the other gland occurs. The fact 

 that section of one splanchnic diminishes the discharge of epine- 

 phrin by a half, without causing any material fall of blood pressure, 

 affords additional evidence that the epinephrin discharged by the 

 adrenal veins is not directly a factor, or at least not an important 

 one in maintaining the blood pressure. 



50 (1228) 



The proportion in which adrenalin distributes itself between cor- 

 puscles and serum in relation to the technique of testing for 

 epinephrin in blood. 



By G. N. Stewart and J. M. Rogoff. 



[From the H. K. Cashing Laboratory of Experimental Medicine of 

 Western Reserve University, Cleveland, Ohio.] 



1. When adrenalin was added to defibrinated blood, and the 

 blood centrifuged after an hour, the serum was found by the 

 colorimetric method of Folin, Cannon and Denis, to contain 

 practically the whole of the added adrenalin. 



3 c.c. adrenalin solution (Parke, Davis & Co.), corresponding to 2.64 mg. epi- 

 nephrin when assayed colorimetrically, was added to 30 c.c. cat's defibrinated blood. 

 Correcting for the small amount of color given by the serum itself in the test, the 

 amount of adrenalin found in 10 c.c. of the serum separated from the adrenalin blood 

 corresponded to 1.37 mg. epinephrin. The proportion by volume of serum in the 

 blood was 62 per cent. The amount of serum in 30 c.c. of the adrenalin blood would, 

 therefore contain 1.37 X 30 X 62/100 = 2.55 mg. adrenalin, i. e., all the adrenalin 

 added was in the serum. 



2. The same result was obtained by assaying the adrenalin in 

 the serum by injection into a pithed cat (method of Elliott). 

 The serum gave a rise indicating, when compared with that given 

 by a known amount of adrenalin in control serum, that 10 c.c. of 

 it contained 1.32 mg. adrenalin. This compares with 1.37 mg. 

 by the colorimetric method. The adrenalin blood gave a rise of 

 blood pressure less than that given by the serum and corresponding 

 to the concentration of adrenalin in it. The sediment, which of 



