112 



Scientific Proceedings (82). 



The above mentioned authors do not state in their preliminary 

 paper how old their cultures were when they made their observa- 

 tions and if they have distinguished between cultures of fat con- 

 taining bone marrow, nearly fatless bone marrow and bone marrow 

 with large amounts of red blood corpuscles. All these facts may 

 alter the conclusions because if the bone marrow contains a large 

 amount of fat then many " Riesenzellen " are present and phago- 

 tization can be observed in a very considerable degree. If fatless 

 bone marrow is used, phagotization appears to go on in a remark- 

 able degree only in the second outlined period of culture life, 

 because only then the cells near the bone marrow network begin 

 to migrate in the plasma clot, to phagotize and to assume different 

 types of connective tissue cells. 



67 (1245) 



On the isolation of streptococci from rabbits. 



Edgar T. H. Tsen (by invitation). 



[From the Department of Bacteriology of the College of Physicians 

 and Surgeons, New York.] 



In our work for the purpose of investigating the relation of 

 streptococci to poliomyelitis as claimed by Dr. E. C. Rosenow, 

 we have made cultures of the brains of 6 monkeys and 20 rabbits. 

 Our technique was as follows: 



The animals were etherized just before death and, when 

 anesthetized, were fastened to an autopsy-board — abdomen down- 

 ward. With animals that died during the night this part of the 

 procedure could not, of course, be carried out. Our purpose was 

 to make sure, whenever possible, that any organisms cultivated 

 from the brains were not post-mortem invaders, but were present 

 during the life of the animal. A median incision was made through 

 the skin over the skull running from the tip of the nose to the 

 back of the neck, and the skin dissected back on both sides of the 

 head. The skull was disinfected with tincture of iodine, and the 

 head and body covered with 3 layers of gauze soaked in lysol. 

 A small hole was made in the gauze so as to expose the upper 



