Isolation of Streptococci from Rabbits. 



113 



part of the skull. The skull was next opened, and, to further 

 insure sterility, the surface of the brain was seared with a scalpel. 

 A big piece, and sometimes one-half of the brain was removed with 

 a pair of long forceps and then immediately put into a Rosenow 

 sterile air chamber, where it was emulsified. (The chamber was 

 sterilized in the hot-air sterilizer for one hour at 160 0 C.) In 

 some cases it was possible to remove considerable parts of the 

 brain while the animal was still alive. The emulsion was poured 

 into a large tube from which different media were inoculated 

 with sterile pipettes. The tubes were examined after two days 

 and, if they were found to be sterile, were again examined two or 

 three days later. The cultures were all kept at 37. 5 0 C. 



The media used were plain broth, ascites broth, glucose broth, 

 glucose ascites broth, plain broth with tissue, ascites broth with 

 tissue, glucose broth with tissue, glucose ascites broth with tissue, 

 glucose agar, and glucose ascites agar. The amount of glucose in 

 the broth was 0.2 per cent, and that in the agar 1 per cent. The 

 reaction of all the media was 0.6 + to 0.8 +. 



A part of the tubes inoculated in each case was prepared by 

 methods simulating as exactly as possible those employed by 

 Dr. E. C. Rosenow. 



All the six monkeys were inoculated with glycerinated polio- 

 myelitis virus — 4 intracerebrally, I subcutaneously, and 1 both 

 intracerebrally and intraperitoneally. Both anaerobic and aerobic 

 cultures of the brain and cord emulsions were made on ascites 

 glucose agar slants at the time of inoculation. We found no 

 growth in any of the tubes. These monkeys were found dead 

 or were etherized from 4 to 16 days after inoculation. Those 4 

 which were inoculated intracerebrally showed typical changes of 

 poliomyelitis and the other two did not. 



From all these 6 monkeys we have isolated streptococci. On 

 November 12, 1916, two rabbits were inoculated intravenously 

 with a 20-hour culture of streptococci isolated from the brain 

 of a monkey which showed typical changes of poliomyelitis. Before 

 inoculation this culture was stated by Dr. Rosenow to be similar 

 to his. Rabbit 1 151 was inoculated with the growth from 22.5 

 c.c. of ascites glucose broth suspended in 1.5 c.c. of sterile salt 



